• Featured Product
  • KD/KO Validated

CD44 Polyclonal antibody

CD44 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)


WB, Neutralization, IP, IHC, IF, FC, CoIP, ELISA



Cat no : 15675-1-AP


CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

Tested Applications

Positive WB detected inMDA-MB-231 cells, HeLa cells, HUVEC cells, A549 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, human colon cancer tissue, A375 cells, HUVEC cells, UCMSCs cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

15675-1-AP targets CD44 in WB, Neutralization, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, sika deer, Bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD44 fusion protein Ag8143
Full Name CD44 molecule (Indian blood group)
Calculated molecular weight 742 aa, 82 kDa
Observed molecular weight 80-95 kDa
GenBank accession numberBC004372
Gene symbol CD44
Gene ID (NCBI) 960
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.


Product Specific Protocols
WB protocol for CD44 antibody 15675-1-APDownload protocol
IHC protocol for CD44 antibody 15675-1-APDownload protocol
IF protocol for CD44 antibody 15675-1-APDownload protocol
IP protocol for CD44 antibody 15675-1-APDownload protocol
FC protocol for CD44 antibody 15675-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




ADAR1 masks the cancer immunotherapeutic promise of ZBP1-driven necroptosis.

Authors - Ting Zhang

Nat Commun

Hyperthermia-triggered biomimetic bubble nanomachines

Authors - Junbin Gao

Adv Sci (Weinh)

The Critical Role of The Piezo1/β-catenin/ATF4 Axis on The Stemness of Gli1+ BMSCs During Simulated Microgravity-Induced Bone Loss

Authors - Yuxiang Hu

Int J Oral Sci

LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma

Authors - Yuantong Liu

Acta Pharm Sin B

Engineering cannabidiol synergistic carbon monoxide nanocomplexes to enhance cancer therapy via excessive autophagy

Authors - Chang Xiao


Hyaluronan decorated layer-by-layer assembled lipid nanoparticles for miR-181a delivery in glioblastoma treatment

Authors - Victor Passos Gibson


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Kishor (Verified Customer) (06-23-2019)

This antibody works very good at 1:1000 in WB over night at 4o C and 1:200 for IF in cultured cells.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 and 1:200
  • Cell Tissue Type: Human cell lines