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CD44 Monoclonal antibody

CD44 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig and More (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

6F4H2

Cat no : 60224-1-Ig

Synonyms

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I



Tested Applications

Positive WB detected inMDA-MB-231 cells, human spleen tissue, HeLa cells, A549 cells, U-251 cells, HUVEC cells, pig spleen tissue
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, human oesophagus cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman lung cancer tissue
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

60224-1-Ig targets CD44 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD44 fusion protein Ag7633
Full Name CD44 molecule (Indian blood group)
Calculated molecular weight 742 aa, 82 kDa
Observed molecular weight 80-95 kDa
GenBank accession numberBC004372
Gene symbol CD44
Gene ID (NCBI) 960
RRIDAB_11042767
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.

Protocols

Product Specific Protocols
WB protocol for CD44 antibody 60224-1-IgDownload protocol
IHC protocol for CD44 antibody 60224-1-IgDownload protocol
IF protocol for CD44 antibody 60224-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Nature

Cell transcriptomic atlas of the non-human primate Macaca fascicularis.

Authors - Lei Han
humanIF

Nat Methods

Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).

Authors - Marine H Laporte
humanIF

Biomater Sci

Magnetothermal heating facilitates the cryogenic recovery of stem cell-laden alginate-Fe(3)O(4) nanocomposite hydrogels

Authors - Xiaozhang Zhang
humanIF

Biomater Sci

Vitrification of stem cell-laden core-shell microfibers with unusually low concentrations of cryoprotective agents

Authors - Conghui Tian
ratIF

Stem Cell Res Ther

Mesenchymal stem cells in synovial fluid increase in number in response to synovitis and display more tissue-reparative phenotypes in osteoarthritis

Authors - Hideto Furuoka
mouseWB

J Nanobiotechnology

Bone-targeted erythrocyte-cancer hybrid membrane-camouflaged nanoparticles for enhancing photothermal and hypoxia-activated chemotherapy of bone invasion by OSCC.

Authors - Hongying Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Udesh (Verified Customer) (08-16-2023)

Worked well for WB at 1:1000

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Glioblastoma cells