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CD44 Monoclonal antibody
CD44 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 60224-1-Ig
Validation Data Gallery
|Positive WB detected in
|MDA-MB-231 cells, human spleen tissue, HeLa cells, A549 cells, U-251 cells, HUVEC cells, pig spleen tissue
|Positive IHC detected in
|human lung cancer tissue, human breast cancer tissue, human oesophagus cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|human lung cancer tissue
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IHC : 1:50-1:500
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
60224-1-Ig targets CD44 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, pig samples.
|human, mouse, rat, rabbit, pig
|Host / Isotype
|Mouse / IgG2a
|CD44 fusion protein Ag7633
|CD44 molecule (Indian blood group)
|Calculated molecular weight
|742 aa, 82 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Udesh (Verified Customer) (08-16-2023)
Worked well for WB at 1:1000