SPARC Polyclonal antibody

• Featured Product
• KD/KO Validated

SPARC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (2)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

41

Cat no : 15274-1-AP

Synonyms

Basement membrane protein 40, BM 40, ON, Osteonectin, SPARC

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Validation Data Gallery

• 

  WB analysis of ROS1728 using 15274-1-AP

  A375 cells, ROS1728 cells, and human placenta tissue were subjected to SDS PAGE followed by western blot with 15274-1-AP (SPARC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 15274-1-AP

  WB result of SPARC antibody (15274-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPARC transfected A375 cells.

• 

  WB analysis using 15274-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15274-1-AP (SPARC antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15274-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15274-1-AP (SPARC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 15274-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15274-1-AP (SPARC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15274-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15274-1-AP (SPARC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15274-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15274-1-AP (SPARC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15274-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15274-1-AP (SPARC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A375 cells, human testis tissue, ROS1728 cells, human placenta tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 30 publications below
• IHC: See 10 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Product Information

15274-1-AP targets SPARC in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat, deer, sika deer
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPARC fusion protein Ag7390
• Full Name: secreted protein, acidic, cysteine-rich (osteonectin)
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35-43 kDa
• GenBank Accession Number: BC004974
• Gene Symbol: SPARC
• Gene ID (NCBI): 6678
• RRID: AB_2194961
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Secreted Protein Acidic And Cysteine Rich (SPARC), also known as osteonectin or basement-membrane protein 40 (BM-40), is a secreted protein essential in the calcification of bone. 

What is the molecular weight of SPARC?

The calculated molecular mass of SPARC is 35 kDa. SPARC is a glycoprotein consisting of 303 amino acids in the cytoplasm and 286 amino acids after the signal sequence is cleaved in secretion. 

Where is SPARC expressed?

Osteoblasts secrete SPARC during bone formation, with high levels found in immature bone compared to mature bone that is in homeostasis (PMID: 2440898). SPARC is also secreted in adult mineralized tissues that have high turnover, such as osteoid and dentin, by cells other than osteoblasts. This includes bone marrow progenitor cells and hypertrophic chondrocytes but also endothelial cells and fibroblasts.

Though previously described as being exclusively expressed in mineralized tissue, it is now understood that SPARC is more widely expressed and is associated with high levels of collagen deposition, found in the extracellular matrix (ECM) of a number of tissues.

What is the function of SPARC?

SPARC contains both a collagen-binding domain and a hydroxyapatite (HA) binding region, so is thought to enhance mineralization by binding both collagen and HA crystals, causing the release of calcium ions (PMID: 26851678).

SPARC can bind to a number of different ECM proteins, particularly collagen, and may even influence the assembly of these proteins (PMID: 19798598). It is known to regulate interactions between cells and the ECM, meaning it plays a role in many important processes such as cell migration and proliferation.

What diseases are associated with SPARC?

Overexpression of SPARC has been identified in a number of different types of cancers (PMID: 18849185), although its role may vary. For example, in neuroblastomas SPARC acts as a suppressor but in gliomas, it causes greater invasion of the tumor. Mutations in SPARC can also lead to Osteogenesis Imperfecta, also known as brittle bone disease (PMID: 26027498).

Publications

Species	Application	Title
human	WB	ACS Appl Mater Interfaces; Evidence for Delivery of Abraxane via a Denatured-Albumin Transport System.; Authors - Maichi Hama
human	IHC	Cancer Discov; PMID: 22585996; Authors - Frese Kristopher K KK
human	WB,IF	Theranostics; A 9-kDa matricellular SPARC fragment released by cathepsin D exhibits pro-tumor activity in the triple-negative breast cancer microenvironment.; Authors - Lindsay B Alcaraz
rat	IHC	Clin Cancer Res; The pancreas cancer microenvironment.; Authors - Feig Christine C
human	IF	Int J Cancer; Tissue-resident CXCR4+ macrophage as a poor prognosis signature promotes pancreatic ductal adenocarcinoma progression; Authors - Zhenyu Liao
human	WB	Int J Mol Sci; Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.; Authors - Federica Morani

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