Anticorps Monoclonal anti-Nestin

Nestin Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

Publications

32

CloneNo.

1D11H2

N° de cat : 66259-1-Ig

Synonymes

NES, Nestin

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Galerie de données de validation

• 

  WB analysis of Neuro-2a using 66259-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66259-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 66259-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 66259-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Neuro-2a, cellules HEK-293, cellules PC-12, tissu de cervelet de rat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• FC: See 2 publications below

Informations sur le produit

66259-1-Ig cible Nestin dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nestin
• Masse moléculaire calculée: 177 kDa
• Poids moléculaire observé: 150-170 kDa
• Numéro d’acquisition GenBank: NM_006617
• Symbole du gène: NES
• Identification du gène (NCBI): 10763
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
human		Cell Res; RNA m6A modification orchestrates a LINE-1-host interaction that facilitates retrotransposition and contributes to long gene vulnerability.; Authors - Feng Xiong
mouse	FC	J Allergy Clin Immunol; Ras homolog family member A/Rho-associated protein kinase 1 signaling modulates lineage commitment of mesenchymal stem cells in asthmatic patients through lymphoid enhancer-binding factor 1.; Authors - Xia Ke
rat		Small; Stemness Maintenance and Massproduction of Neural Stem Cells on Poly L-Lactic Acid Nanofibrous Membrane Based on Piezoelectriceffect.; Authors - Xiheng Lu
mouse	WB	EBioMedicine; Silencing of circular RNA HIPK2 in neural stem cells enhances functional recovery following ischaemic stroke.; Authors - Guangtian Wang
rat		Int J Biol Macromol; A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects; Authors - Wentao Shi

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