DACH1 Polyclonal antibody

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  WB analysis using 10914-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10914-1-AP (DACH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10914-1-AP

  WB result of DACH1 (10914-1-AP, 1:4000) with si-control and si-DACH1 transfected HEK293 cells.

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  IP experiment of HEK-293 using 10914-1-AP

  IP result of anti-DACH1 (IP:10914-1-AP, 4ug; Detection:10914-1-AP 1:1000) with HEK-293 cells lysate 6000ug.

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  IHC staining of human oesophagus cancer using 10914-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10914-1-AP (DACH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 10914-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10914-1-AP (DACH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10914-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10914-1-AP (DACH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10914-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10914-1-AP (DACH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 10914-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DACH1 antibody (10914-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

DACH1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10914-1-AP

Publications(76)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Synonyms

DACH, Dachshund-1

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Tested Applications

• Positive WB detected in: H9C2 cells, HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human oesophagus cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 28 publications below
• IHC: See 31 publications below
• IF: See 40 publications below
• IP: See 1 publications below
• ChIP: See 2 publications below

Product Information

10914-1-AP targets DACH1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1354

  Product name: Recombinant human DACH1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 176-521 aa of BC021219

  Sequence: QHGADSENGDMNSSVGLELPFMMMPHPLIPVSLPPASVTMAMSQMNHLSTIANMAAAAQVQSPPSRVETSVIKERVPDSPSPAPSLEEGRRPGSHPSSHRSSSVSSSPARTESSSDRIPVHQNGLSMNQMLMGLSPNVLPGPKEGDLAGHDMGHESKRMHIEKDETPLSTPTARDSLDKLSLTGHGQPLPPGFPSPFLFPDGLSSIETLLTNIQGLLKVAIDNARAQEKQVQLEKTELKMDFLRERELRETLEKQLAMEQKNRAIVQKRLKKEKKAKRKLQEALEFETKRREQAEQTLKQAASTDSLRVLNDSLTPEIEADRSGGRTDAERTIQDGRLYLKTTVMY

• Full Name: dachshund homolog 1 (Drosophila)
• Calculated Molecular Weight: 79 kDa
• Observed Molecular Weight: 97-110 kDa
• GenBank Accession Number: BC021219
• Gene Symbol: DACH1
• Gene ID (NCBI): 1602
• RRID: AB_2230330
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UI36
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells. Dach1 expression is enriched in rECs, which are associated with osteoprogenitors and bone-resorbing osteoclasts, and overexpression of DACH1 in postnatal mice induces a strong increase in arteries and type R capillaries, leading to local metabolic changes and enabling trabecular bone formation in normally highly hypoxic areas of the diaphysis (PMID: 39528700). Moreover, Loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human DACH1.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Nature; Single-cell analysis of early progenitor cells that build coronary arteries.; Authors - Tianying Su
human	IF	Nature; Excitation-induced ataxin-3 aggregation in neurons from patients with Machado-Joseph disease.; Authors - Koch Philipp P
mouse	IF	Science; Breathing control center neurons that promote arousal in mice.; Authors - Kevin Yackle
human	IF	Cell Stem Cell; Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders.; Authors - Carmen Lorenz
mouse	IHC	Nat Cell Biol; A single-cell transcriptional roadmap for cardiopharyngeal fate diversification.; Authors - Wei Wang
human	WB	Nat Neurosci; Auto-attraction of neural precursors and their neuronal progeny impairs neuronal migration.; Authors - Julia Ladewig

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (11-02-2021)

This antibody works great for IF. It specifically marked DACH1-positive coronary vessel cells in mouse heart.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Heart