Tested Applications
| Positive WB detected in | H9C2 cells, HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human oesophagus cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10914-1-AP targets DACH1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1354 Product name: Recombinant human DACH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 176-521 aa of BC021219 Sequence: QHGADSENGDMNSSVGLELPFMMMPHPLIPVSLPPASVTMAMSQMNHLSTIANMAAAAQVQSPPSRVETSVIKERVPDSPSPAPSLEEGRRPGSHPSSHRSSSVSSSPARTESSSDRIPVHQNGLSMNQMLMGLSPNVLPGPKEGDLAGHDMGHESKRMHIEKDETPLSTPTARDSLDKLSLTGHGQPLPPGFPSPFLFPDGLSSIETLLTNIQGLLKVAIDNARAQEKQVQLEKTELKMDFLRERELRETLEKQLAMEQKNRAIVQKRLKKEKKAKRKLQEALEFETKRREQAEQTLKQAASTDSLRVLNDSLTPEIEADRSGGRTDAERTIQDGRLYLKTTVMY Predict reactive species |
| Full Name | dachshund homolog 1 (Drosophila) |
| Calculated Molecular Weight | 79 kDa |
| Observed Molecular Weight | 97-110 kDa |
| GenBank Accession Number | BC021219 |
| Gene Symbol | DACH1 |
| Gene ID (NCBI) | 1602 |
| RRID | AB_2230330 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UI36 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells. Dach1 expression is enriched in rECs, which are associated with osteoprogenitors and bone-resorbing osteoclasts, and overexpression of DACH1 in postnatal mice induces a strong increase in arteries and type R capillaries, leading to local metabolic changes and enabling trabecular bone formation in normally highly hypoxic areas of the diaphysis (PMID: 39528700). Moreover, Loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human DACH1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DACH1 antibody 10914-1-AP | Download protocol |
| IHC protocol for DACH1 antibody 10914-1-AP | Download protocol |
| IP protocol for DACH1 antibody 10914-1-AP | Download protocol |
| WB protocol for DACH1 antibody 10914-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-DACH1 antibody (Cat# 10914-1-AP) has accumulated 86 citations in high-impact journals including Nature, Science, Cell, Neuron, Cancer Research, Nature Cell Biology, Nature Neuroscience, JCI, and Genes & Development. Associated research fields span neuroscience (neural development, neurodegeneration, Alzheimer's disease), oncology (lung, breast, prostate, colorectal, pancreatic, and gastric cancers), cardiology (coronary angiogenesis, diabetic cardiomyopathy), nephrology (podocyte biology, kidney disease), stem cell biology, and developmental biology.
| Species | Application | Title |
|---|---|---|
Nature Excitation-induced ataxin-3 aggregation in neurons from patients with Machado-Joseph disease. | ||
J Hematol Oncol DACH1 antagonizes CXCL8 to repress tumorigenesis of lung adenocarcinoma and improve prognosis. | ||
J Hematol Oncol DACH1 inhibits cyclin D1 expression, cellular proliferation and tumor growth of renal cancer cells. | ||
Science Subventricular zone radial glial cells maintain inhibitory neuron production in the human brain. | ||
Reviews
What customers say
The single review reports excellent performance for immunofluorescence. The antibody specifically marked DACH1-positive coronary vessel cells in mouse heart tissue at a 1:100 dilution. The reviewer rated it 5 out of 5, describing it as working "great" for IF applications. Overall, the feedback is highly positive, confirming strong specificity and reliable staining in mouse heart samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tom (Verified Customer) (11-02-2021) | This antibody works great for IF. It specifically marked DACH1-positive coronary vessel cells in mouse heart.
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