DACH1 Monoclonal antibody

• Featured Product
• KD/KO Validated

DACH1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

3B6D2

Cat no : 60082-1-Ig

Synonyms

Dachshund-1, DACH, 3B6D2

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Validation Data Gallery

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  WB analysis of HEK-293 using 60082-1-Ig

  WB result of DACH1 (60082-1-Ig, 1:5000) with si-control and si-DACH1 transfected HEK293 cells.

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  WB analysis of MCF-7 using 60082-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 60082-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 60082-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 60082-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 60082-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60082-1-Ig (DACH1 antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 60082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 60082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 60082-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60082-1-Ig (DACH1 antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse embryo using 60082-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse embryo using 60082-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 60082-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 60082-1-Ig (DACH1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HEK-293 using 60082-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, C6 cells, L02 cells, ROS1728 cells, HEK-293 cells
• Positive IHC detected in: mouse brain tissue, human endometrial cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse embryo tissue, mouse brain tissue
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:10-1:100
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

60082-1-Ig targets DACH1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DACH1 fusion protein Ag4474
• Full Name: dachshund homolog 1 (Drosophila)
• Calculated Molecular Weight: 79 kDa
• Observed Molecular Weight: 97-110 kDa
• GenBank Accession Number: BC021219
• Gene Symbol: DACH1
• Gene ID (NCBI): 1602
• RRID: AB_2089711
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing, but isoform1(97-110kd) is the predominantly expressed form in tissue. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human DACH1.

Publications

Species	Application	Title
human,mouse	WB,IP,IF	Am J Respir Cell Mol Biol; DACH1 Attenuates Airway Inflammation in COPD by Activating NRF2 Signaling; Authors - Qian Huang
human	WB	Cell Biol Int; DACH1 inhibits the proliferation and migration of papillary thyroid carcinoma; Authors - Shengru Liang
human	WB	Front Oncol; Identification and verification of the temozolomide resistance feature gene DACH1 in gliomas; Authors - Qiang Gu ; KD Validated
human	WB	Clin Exp Metastasis; Lung-derived soluble factors support stemness/plasticity and metastatic behaviour of breast cancer cells via the FGF2-DACH1 axis; Authors - Vasudeva Bhat