Anticorps Polyclonal de lapin anti-Glucocorticoid receptor

• Phare
• Validé par KD/KO

Glucocorticoid receptor Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA

Conjugaison

Non conjugué

Publications

86

Avis (4)

N° de cat : 24050-1-AP

Synonymes

GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1

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Galerie de données de validation

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  WB analysis using 24050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 24050-1-AP

  WB result of Glucocorticoid receptor antibody (24050-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glucocorticoid receptor transfected HepG2 cells.

• 

  WB analysis using 24050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 24050-1-AP

  IP result of anti-Glucocorticoid receptor (IP:24050-1-AP, 4ug; Detection:24050-1-AP 1:4000) with HepG2 cells lysate 1720 ug.

• 

  IP experiment of HepG2 using 24050-1-AP

  IP result of anti-Glucocorticoid receptor (IP:24050-1-AP, 4ug; Detection:24050-1-AP 1:4000) with HepG2 cells lysate 1720 ug.

• 

  IP experiment of mouse heart using 24050-1-AP

  IP result of anti-Glucocorticoid receptor (IP:24050-1-AP, 4ug; Detection:24050-1-AP 1:500) with mouse heart tissue lysate 4400ug.

• 

  IHC staining of human prostate cancer using 24050-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 24050-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 24050-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 24050-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 24050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glucocorticoid receptor antibody (24050-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 24050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Glucocorticoid receptor antibody (24050-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 24050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glucocorticoid receptor antibody (24050-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules DU 145, cellules HeLa, cellules HepG2, cellules PC-12, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HepG2, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 11 publications below
• WB: See 62 publications below
• IHC: See 6 publications below
• IF: See 15 publications below
• IP: See 2 publications below
• CoIP: See 5 publications below
• ChIP: See 17 publications below

Informations sur le produit

24050-1-AP cible Glucocorticoid receptor dans les applications de WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Glucocorticoid receptor Protéine recombinante Ag21146
• Nom complet: nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 94-97 kDa
• Numéro d’acquisition GenBank: BC015610
• Symbole du gène: Glucocorticoid receptor
• Identification du gène (NCBI): 2908
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).

Publications

Species	Application	Title
human	WB	Cell Metab; Individual-specific functional epigenomics reveals genetic determinants of adverse metabolic effects of glucocorticoids.; Authors - Wenxiang Hu
mouse	IHC	Cell Metab; A macrophage-hepatocyte glucocorticoid receptor axis coordinates fasting ketogenesis.; Authors - Anne Loft
human	WB	Sci Adv; Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment.; Authors - Leiming Wang
mouse	chIP	Mol Cell; Cistromic Reprogramming of the Diurnal Glucocorticoid Hormone Response by High-Fat Diet.; Authors - Fabiana Quagliarini
mouse	WB,ChIP,IP	J Clin Invest; REVERBa couples the circadian clock to hepatic glucocorticoid action.; Authors - Giorgio Caratti
mouse	ChIP	Mol Psychiatry; The co-chaperone Fkbp5 shapes the acute stress response in the paraventricular nucleus of the hypothalamus of male mice.; Authors - Alexander S Häusl

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ping (Verified Customer) (06-04-2019)

This antibody works well for immunoprecipitation and western blot.

• Applications: Western Blot, Immunoprecipitation,
• Cell Tissue Type: mouse brain tissue

FH

Yu Siong (Verified Customer) (04-23-2019)

Multiple bands observed - Total of 5 bands, not suitable for porcine brain endothelial cells, as non-specific binding tend to occur.Consistent in two Independent experiments

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Porcine brain endothelial cells

FH

Praveen (Verified Customer) (02-25-2019)

WORKS VERY WELL.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Cell culture, ChIP,
• Cell Tissue Type: Ac16

FH

Shubham (Verified Customer) (01-31-2019)

Antibody works great.

• Applications: Western Blot, Immunohistochemistry, Cell culture,
• Primary Antibody Dilution: 1:10
• Cell Tissue Type: Ac16

Glucocorticoid receptor Antibody Western Blot,Immunohistochemistry,Cell culture, validation (1:10 dilution) in Ac16 (Cat no:24050-1-AP)

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