Validation Data Gallery
|Positive WB detected in||HeLa cells, UV treated HeLa cells, Jurkat cells, MCF-7 cells, HEK-293 cells, RAW264.7, HT-1080 cells, HL-60 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human testis tissue, human ovary tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 19064-1-AP is BAG1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BAG1 fusion protein Ag13555|
|Full Name||BCL2-associated athanogene|
|Calculated molecular weight||39 kDa|
|Observed molecular weight||50-55 kDa, 30-33 kDa|
|GenBank accession number||BC014774|
|Gene ID (NCBI)||573|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
BAG1 have been identified that modulate gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody can recognize all the isoforms of BAG1.
|Product Specific Protocols|
|WB protocol for BAG1 antibody 19064-1-AP||Download protocol|
|IHC protocol for BAG1 antibody 19064-1-AP||Download protocol|
|IF protocol for BAG1 antibody 19064-1-AP||Download protocol|
|IP protocol for BAG1 antibody 19064-1-AP||Download protocol|
|Click here to view our Standard Protocols|
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tongbin (Verified Customer) (08-25-2020)
This BAG1 antibody works very well in detecting endogenous BAG1 protein by western blots.