Progesterone Receptor Monoclonal antibody

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  IHC staining of human breast cancer using 66300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66300-1-Ig (Progesterone Receptor antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66300-1-Ig (Progesterone Receptor antibody) at dilution of 1:9900 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66300-1-Ig (Progesterone Receptor antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Progesterone Receptor Monoclonal Antibody for IHC, ELISA

Cat No. 66300-1-Ig

Clone No.3D1D12

Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, ELISA

Synonyms

PGR, PRGR, PR, NR3C3, 3D1D12

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Tested Applications

• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:5000-1:15000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

66300-1-Ig targets Progesterone Receptor in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Progesterone Receptor fusion protein Ag22992
• Full Name: progesterone receptor
• Calculated Molecular Weight: 933 aa, 99 kDa
• GenBank Accession Number: NM_000926
• Gene Symbol: Progesterone Receptor
• Gene ID (NCBI): 5241
• RRID: AB_2881683
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P06401
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGR (Progesterone Receptor), also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. The ER and PR status has been used as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence.

Publications

Species	Application	Title
human	IHC	Ann Med; Organoids from patient biopsy samples can predict the response of BC patients to neoadjuvant chemotherapy; Authors - Dan Shu
human	IHC	Mol Med Rep; HPK1 positive expression associated with longer overall survival in patients with estrogen receptor-positive invasive ductal carcinoma‑not otherwise specified.; Authors - Jiaojiao Wang