FKBP52 Polyclonal antibody

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• FKBP52 Polyclonal antibody (10655-1-AP)

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FKBP52 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10655-1-AP

Publications(22)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

FKBP4, 51 kDa FK506-binding protein, 52 kDa FK506-binding protein, EC:5.2.1.8, FK506-binding protein 4

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  WB analysis using 10655-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 10655-1-AP

  WB result of FKBP52 antibody (10655-1-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP52 transfected MCF-7 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 10655-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 10655-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 10655-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 10655-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 10655-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 10655-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 10655-1-AP

  IP result of anti-FKBP52 (IP:10655-1-AP, 4ug; Detection:10655-1-AP 1:20000) with HeLa cells lysate 1360 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 10655-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10655-1-AP (FKBP52 antibody) at dilution of 1:100 (under 25x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 10655-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FKBP52 antibody (10655-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, PC-3 cells, NIH/3T3 cells, Jurkat cells, MCF-7 cells, HSC-T6 cells, C6 cells, rat testis tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10655-1-AP targets FKBP52 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1019

  Product name: Recombinant human FKBP52 protein

  Source: e coli.-derived, PKG

  Tag: GST

  Domain: 1-286 aa of BC007924

  Sequence: MTAEEMKATESGAQSAPLPMEGVDISPKQDEGVLKVIKREGTGTEMPMIGDRVFVHYTGWLLDGTKFDSSLDRKDKFSFDLGKGEVIKAWDIAIATMKVGEVCHITCKPEYAYGSAGSPPKIPPNATLVFEVELFEFKGEDLTEEEDGGIIRRIQTRGEGYAKPNEGAIVEVALEGYYKDKLFDQRELRFEIGEGENLDLPYGLERAIQRMEKGEHSIVYLKPSYAFGSVGKEKFQIPPNAELKYELHLKSFEKAKESWEMNSEEKLEQSTIVKERGTVYFKEGKY

• Full Name: FK506 binding protein 4, 59kDa
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC007924
• Gene Symbol: FKBP52
• Gene ID (NCBI): 2288
• RRID: AB_2246853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q02790
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FKBP52 (FK506 binding protein 4) is a member of the immunophilin protein family that comprises intracellular protein effectors of immunosuppressive drugs, and it is known as a steroid receptor-associated protein. As a novel regulator of microtubule dynamics, FKBP52 is associated with the motor protein dynein and with the cytoskeleton during mitosis. FKBP52 is highly expressed in CNS regions susceptible to Alzheimer's, and plays a role in modulating toxicity of Abeta peptides. The protein can associate with the Tau function, and may help to decipher and modulate the events involved in Tau-induced neurodegeneration. In addition, FKBP52 is likely to play a role in growth and development of the male genitalia.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Discov; Lysine butyrylation of HSP90 regulated by KAT8 and HDAC11 confers chemoresistance; Authors - Yan He
human	WB	Theranostics; FKBP4 connects mTORC2 and PI3K to activate the PDK1/Akt-dependent cell proliferation signaling in breast cancer.; Authors - Alain Mangé ; KD Validated
human	WB,IHC	Clin Cancer Res; Identification of a new panel of serum autoantibodies associated with the presence of in situ carcinoma of the breast in younger women.; Authors - Desmetz Caroline C
		Int J Biol Macromol; FKBP4-mediated reprogramming of H3K4bhb at energy metabolism ameliorates ischemic acute kidney injury.; Authors - Zhibo Zhao
mouse,human	WB	Antioxidants (Basel); Chlorogenic Acid Alleviated AFB1-Induced Hepatotoxicity by Regulating Mitochondrial Function, Activating Nrf2/HO-1, and Inhibiting Noncanonical NF-κB Signaling Pathway; Authors - Qianqian Wang
human	WB	Front Immunol; Naringenin Regulates FKBP4/NR3C1/NRF2 Axis in Autophagy and Proliferation of Breast Cancer and Differentiation and Maturation of Dendritic Cell.; Authors - Hanchu Xiong ; KD Validated