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FKBP52 Polyclonal antibody
FKBP52 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 10655-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, PC-3 cells, Jurkat cells, NIH/3T3 cells, MCF-7 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 5 publications below |
WB | See 11 publications below |
IHC | See 4 publications below |
IP | See 1 publications below |
Product Information
10655-1-AP targets FKBP52 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, chicken |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FKBP52 fusion protein Ag1019 |
Full Name | FK506 binding protein 4, 59kDa |
Calculated molecular weight | 52 kDa |
Observed molecular weight | 52 kDa |
GenBank accession number | BC007924 |
Gene symbol | FKBP4 |
Gene ID (NCBI) | 2288 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FKBP52 (FK506 binding protein 4) is a member of the immunophilin protein family that comprises intracellular protein effectors of immunosuppressive drugs, and it is known as a steroid receptor-associated protein. As a novel regulator of microtubule dynamics, FKBP52 is associated with the motor protein dynein and with the cytoskeleton during mitosis. FKBP52 is highly expressed in CNS regions susceptible to Alzheimer's, and plays a role in modulating toxicity of Abeta peptides. The protein can associate with the Tau function, and may help to decipher and modulate the events involved in Tau-induced neurodegeneration. In addition, FKBP52 is likely to play a role in growth and development of the male genitalia.
Protocols
Product Specific Protocols | |
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WB protocol for FKBP52 antibody 10655-1-AP | Download protocol |
IHC protocol for FKBP52 antibody 10655-1-AP | Download protocol |
IF protocol for FKBP52 antibody 10655-1-AP | Download protocol |
IP protocol for FKBP52 antibody 10655-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Theranostics FKBP4 connects mTORC2 and PI3K to activate the PDK1/Akt-dependent cell proliferation signaling in breast cancer.
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Clin Cancer Res Identification of a new panel of serum autoantibodies associated with the presence of in situ carcinoma of the breast in younger women. | ||
Mol Oncol FKBP51 and FKBP52 regulate androgen receptor dimerization and proliferation in prostate cancer cells.
| ||
J Cell Physiol Multiple roles for peptidylglycine α-amidating monooxygenase in the response to hypoxia. | ||
RNA The co-chaperones Fkbp4/5 control Argonaute2 expression and facilitate RISC assembly.
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Front Immunol Naringenin Regulates FKBP4/NR3C1/NRF2 Axis in Autophagy and Proliferation of Breast Cancer and Differentiation and Maturation of Dendritic Cell.
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