HSP90 Polyclonal antibody

• Featured Product
• KD/KO Validated

HSP90 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

312

Reviews (5)

Cat no : 13171-1-AP

Synonyms

HSP90AA1, HSP90A, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90 alpha, Heat shock protein family C member 1

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Validation Data Gallery

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  WB analysis using 13171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13171-1-AP (HSP90 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13171-1-AP (HSP90 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 13171-1-AP

  IP result of anti-HSP90 (IP:13171-1-AP, 3ug; Detection:13171-1-AP 1:500) with K-562 cells lysate 2000ug.

• 

  IP experiment of HeLa using 13171-1-AP

  IP result of anti-HSP90 (IP:13171-1-AP, 3ug; Detection:13171-1-AP 1:500) with HeLa cells lysate 4400ug.

• 

  IHC staining of mouse testis using 13171-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13171-1-AP (HSP90 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13171-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13171-1-AP (HSP90 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 13171-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13171-1-AP (HSP90 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13171-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP90 antibody (13171-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 13171-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP90 antibody (13171-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 13171-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug HSP90 Polyclonal antibody (13171-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, PC-12 cells, C6 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue
• Positive IP detected in: K-562 cells, HeLa cells
• Positive IHC detected in: mouse testis tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1600-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 281 publications below
• IHC: See 13 publications below
• IF: See 17 publications below
• IP: See 14 publications below
• ELISA: See 3 publications below
• CoIP: See 15 publications below
• RIP: See 3 publications below

Product Information

13171-1-AP targets HSP90 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, bovine, sheep, fish, carp
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSP90 fusion protein Ag3826
• Full Name: heat shock protein 90kDa alpha (cytosolic), class A member 1
• Calculated Molecular Weight: 853 aa, 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC023006
• Gene Symbol: HSP90
• Gene ID (NCBI): 3320
• RRID: AB_2120924
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.

Publications

Species	Application	Title
human	WB	Nature; CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.; Authors - Lingyun Long
mouse	WB	Cell Metab; IgG is an aging factor that drives adipose tissue fibrosis and metabolic decline; Authors - Lexiang Yu
	CoIP	Nat Cell Biol; LIMIT is an immunogenic lncRNA in cancer immunity and immunotherapy.; Authors - Gaopeng Li
mouse	WB	Bioact Mater; Genetically engineered M2-like macrophage-derived exosomes for P. gingivalis-suppressed cementum regeneration: From mechanism to therapy; Authors - Xin Huang
mouse	WB	Nat Commun; The CUL5 E3 ligase complex negatively regulates central signaling pathways in CD8+ T cells; Authors - Xiaofeng Liao
mouse,rat	WB	Nat Commun; Protective effects of Pt-N-C single-atom nanozymes against myocardial ischemia-reperfusion injury; Authors - Tianbao Ye

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S (Verified Customer) (08-14-2024)

excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: hek

HSP90 Antibody Western Blot validation (1000 dilution) in hek (Cat no:13171-1-AP)

FH

Satyendra (Verified Customer) (07-06-2022)

Works very nicely with CEF, DF-1 and VeroE6 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: VeroE6 cells

FH

Guorong (Verified Customer) (03-22-2022)

Excellent performance with a specific band of expected size

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: ARPE-19

HSP90 Antibody Western Blot validation (1:2500 dilution) in ARPE-19 (Cat no:13171-1-AP)

FH

Ekaterini (Verified Customer) (02-16-2022)

The best antibody to use for loading control. Always clear and it is not affected by drug treatments.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ALL cell lines

FH

Xiaoping (Verified Customer) (10-22-2018)

This antibody works well in iPS, iMns or MEF. The signal is pretty strong which is better than the one we used before and the background is very low.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human neuron and mouse fibroblast samples

HSP90 Antibody Western Blot, validation (1:1000 dilution) in Human neuron and mouse fibroblast samples (Cat no:13171-1-AP)

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