Anticorps Polyclonal de lapin anti-Nestin

Nestin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

83

Avis (4)

N° de cat : 19483-1-AP

Synonymes

NES

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Galerie de données de validation

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  WB analysis using 19483-1-AP

  Mouse brain lysates were subjected to SDS PAGE followed by western blot with 19483-1-AP (Nestin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 19483-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 19483-1-AP (NES antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human kidney using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:400 (under 40x lens).

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  IF Staining of U-251 using 19483-1-AP

  Immunofluorescent analysis of U-251 cells using 19483-1-AP (Nestin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SH-SY5Y using 19483-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Nestin antibody (19483-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SH-SY5Y using 19483-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 19483-1-AP (Nestin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu rénal humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y, cellules U-251

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 28 publications below
• IHC: See 10 publications below
• IF: See 55 publications below
• FC: See 1 publications below

Informations sur le produit

19483-1-AP cible Nestin dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nestin
• Masse moléculaire calculée: 177 kDa
• Poids moléculaire observé: 170-180 kDa
• Numéro d’acquisition GenBank: NM_006617
• Symbole du gène: Nestin
• Identification du gène (NCBI): 10763
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).

Publications

Species	Application	Title
rat	IF	ACS Nano; Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury; Authors - Weidong Wu
	IF	J Biomed Sci; Genetic and pharmacologic p32-inhibition rescue CHCHD2-linked Parkinson's disease phenotypes in vivo and in cell models; Authors - Murni Tio
human	WB,IF	Neuro Oncol; TRIM24 Promotes Stemness and Invasiveness of Glioblastoma Cells via Activating SOX2 Expression.; Authors - Lu-Hua Zhang
human,mouse	IF	Redox Biol; MG53 protein rejuvenates hUC-MSCs and facilitates their therapeutic effects in AD mice by activating Nrf2 signaling pathway.; Authors - Shanshan Ma
rat	IF	Neuropsychopharmacology; Exosomes from patients with major depression cause depressive-like behaviors in mice with involvement of miR-139-5p-regulated neurogenesis.; Authors - Ze-Xu Wei
rat	IF	J Mater Chem B; Integration of genetically engineered virus nanofibers and fibrin to form injectable fibrous neuron-rich hydrogels and enable neural differentiation; Authors - Yingfan Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Reyes (Verified Customer) (03-18-2024)

I tested nestin (in red) in human brain cortical sections obtained from pediatric patients, and the result was inconclusive, as it looks like ther is a halo around my DAPI, and there is no clear nestin marking in any cell type in the tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

Nestin Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:19483-1-AP)

FH

Udesh (Verified Customer) (02-24-2023)

Nestin in the upper lane Worked well at 1:1000 in 5% BSA, O/N, 4 degrees

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS Osteosarcoma cells

Nestin Antibody Western Blot validation (1:1000 dilution) in U2OS Osteosarcoma cells (Cat no:19483-1-AP)

FH

Balawant (Verified Customer) (08-23-2022)

this antibody is excellent for westernblot

• Applications: Western Blot
• Cell Tissue Type: colon tissue and colon cell line

FH

Silvia (Verified Customer) (09-30-2021)

Immunofluorescence (1:200) works well on NPC

• Applications: Immunofluorescence

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