Anticorps Polyclonal de lapin anti-ND1

• Phare
• Validé par KD/KO

ND1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

77

Avis (3)

N° de cat : 19703-1-AP

Synonymes

MT ND1, MTND1, NADH dehydrogenase subunit 1, NADH1, ND1

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Galerie de données de validation

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  WB analysis using 19703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19703-1-AP (ND1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 19703-1-AP

  IP result of anti-ND1 (IP:19703-1-AP, 3ug; Detection:19703-1-AP 1:500) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human liver using 19703-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19703-1-AP (ND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 19703-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19703-1-AP (ND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, mouse liver mitochondria
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 74 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

19703-1-AP cible ND1 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: NADH dehydrogenase, subunit 1 (complex I)
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 28-38 kDa
• Numéro d’acquisition GenBank: NC_012920
• Symbole du gène: ND1
• Identification du gène (NCBI): 4535
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ND1 belongs to the complex I subunit 1 family. ND1 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Publications

Species	Application	Title
human	IF	Cell; Single-Cell Transcriptomic Atlas of Primate Ovarian Aging.; Authors - Si Wang
mouse	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang ; KD Validated
mouse	IHC	Circulation; Extracellular Tuning of Mitochondrial Respiration Leads to Aortic Aneurysm.; Authors - Jorge Oller
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
human	WB	Sci Adv; MCART1/SLC25A51 is required for mitochondrial NAD transport.; Authors - Nora Kory
mouse	WB	Nat Commun; Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.; Authors - Zhisheng Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tania (Verified Customer) (03-05-2022)

a lot of background. difficult to say each band is the correct one.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ES2 human cells

FH

Daniel (Verified Customer) (10-01-2019)

ND2 was detected in both liver (L) and heart (H) lysate from a wildtype mouse. No ND1 was detected in samples processed in parallel.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver (L), heart (H)

ND1 Antibody Western Blot, validation (1:1000 dilution) in Mouse liver (L), heart (H) (Cat no:19703-1-AP)

FH

Loukmane (Verified Customer) (08-10-2018)

It works very well with A549 and lung tissue, although with the former the background is higherThe image shows ND1 in whole A549 lysate

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Lung tissue and A549

ND1 Antibody Western Blot, validation (1:500 dilution) in Lung tissue and A549 (Cat no:19703-1-AP)

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