NDUFA9 Polyclonal antibody
NDUFA9 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 20312-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, mouse heart tissue |
Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 23 publications below |
IHC | See 1 publications below |
Product Information
20312-1-AP targets NDUFA9 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, pig, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NDUFA9 fusion protein Ag14150 |
Full Name | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa |
Calculated Molecular Weight | 377 aa, 43 kDa |
Observed Molecular Weight | 35 kDa |
GenBank Accession Number | BC009311 |
Gene Symbol | NDUFA9 |
Gene ID (NCBI) | 4704 |
RRID | AB_10694678 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NDUFA9(NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial) is also named as NDUFS2L, CI-39kD and belongs to the complex I NDUFA9 subunit family. It is involved in stabilizing the junction between membrane and matrix arms of complex I, a late assembly step critical for complex I biogenesis and activity.
Protocols
Product Specific Protocols | |
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WB protocol for NDUFA9 antibody 20312-1-AP | Download protocol |
IHC protocol for NDUFA9 antibody 20312-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
EMBO J EglN2 associates with the NRF1-PGC1α complex and controls mitochondrial function in breast cancer. | ||
Dev Cell Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells. | ||
J Cell Biol FAM92A1 is a BAR domain protein required for mitochondrial ultrastructure and function. | ||
Cell Mol Life Sci GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation | ||