NDUFS3 Polyclonal antibody

• Featured Product
• KD/KO Validated

NDUFS3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

31

Cat no : 15066-1-AP

Synonyms

MC1DN8, EC:7.1.1.2, Complex I-30kD, CI-30kD, CI30kD

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Validation Data Gallery

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  WB analysis using 15066-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15066-1-AP (NDUFS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15066-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15066-1-AP (NDUFS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15066-1-AP

  IP result of anti-NDUFS3 (IP:15066-1-AP, 4ug; Detection:15066-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of mouse testis using 15066-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15066-1-AP (NDUFS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15066-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NDUFS3 antibody (15066-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HepG2 cells, mouse brain tissue, mouse heart tissue, rat heart tissue, rat brain tissue, NIH/3T3 cells
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 26 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Product Information

15066-1-AP targets NDUFS3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFS3 fusion protein Ag7072
• Full Name: NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC000617
• Gene Symbol: NDUFS3
• Gene ID (NCBI): 4722
• RRID: AB_2151109
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDUFS3(NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial) is also named as CI-30kD and belongs to the complex I 30 kDa subunit family. It catalyzes the oxidation of NADH, with the concomitant reduction of ubiquinone and ejection of protons out of the mitochondria(PMID:10967146). NDUFS3 can be used as a mitochondrial matrix control(PMID:17344420). The full length has a transit peptide with 36 amino acids.

Publications

Species	Application	Title
human	WB,IHC	Cancer Discov; NCOA4-Mediated Ferritinophagy Is a Pancreatic Cancer Dependency via Maintenance of Iron Bioavailability for Iron-Sulfur Cluster Proteins; Authors - Naiara Santana-Codina
human	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
human	WB	EMBO Rep; AIF inhibits tumor metastasis by protecting PTEN from oxidation.; Authors - Shao-Ming Shen
human	WB	J Cell Biol; PDGFRβ translocates to the nucleus and regulates chromatin remodeling via TATA element-modifying factor 1.; Authors - Natalia Papadopoulos
mouse	WB	Diabetes; DW14006 as a Direct AMPKα Activator Ameliorates Diabetic Peripheral Neuropathy in Mice.; Authors - Xu Xu
human	WB	J Med Genet; COA7 (C1orf163/RESA1) mutations associated with mitochondrial leukoencephalopathy and cytochrome c oxidase deficiency.; Authors - Anabel Martinez Lyons