NDUFV1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NDUFV1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

44

Cat no : 11238-1-AP

Synonyms

CI 51kD, Complex I 51kD, NDUFV1, UQOR1

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Validation Data Gallery

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  WB analysis using 11238-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11238-1-AP (NDUFV1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 11238-1-AP

  WB result of NDUFV1 antibody (11238-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFV1 transfected A549 cells.

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  IP experiment of A431 using 11238-1-AP

  IP result of anti-NDUFV1 (IP:11238-1-AP, 4ug; Detection:11238-1-AP 1:500) with A431 cells lysate 2000ug.

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  IHC staining of human lung cancer using 11238-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11238-1-AP (NDUFV1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 11238-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11238-1-AP (NDUFV1 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HeLa cells, HepG2 cells, U2OS cells, mouse heart tissue
• Positive IP detected in: A431 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 42 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

11238-1-AP targets NDUFV1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFV1 fusion protein Ag1764
• Full Name: NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC015645
• Gene Symbol: NDUFV1
• Gene ID (NCBI): 4723
• RRID: AB_2149040
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DUFV1(NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial) is also named as UQOR1,Complex I-51kD and belongs to the complex I 51 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. It has 2 isoforms produced by alternative splicing. Defects in NDUFV1 are a cause of Leigh syndrome (LS) and mitochondrial complex I deficiency (MT-C1D).

Publications

Species	Application	Title
human	WB	Nature; In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer.; Authors - Milica Momcilovic
human	WB	Nature; Accessory subunits are integral for assembly and function of human mitochondrial complex I.; Authors - David A Stroud
human	WB	Mol Cell; Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex.; Authors - Yilin Kang
human,mouse	WB	Nat Commun; A salvage pathway maintains highly functional respiratory complex I.; Authors - Karolina Szczepanowska
mouse	WB	Brain; CLPP deficiency ameliorates neurodegeneration caused by impaired mitochondrial protein synthesis.; Authors - Anastasia Rumyantseva
mouse	WB	Dev Cell; Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells.; Authors - Rani Ojha