TST Polyclonal antibody

TST Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 16311-1-AP

Synonyms

RDS, Rhodanese, Thiosulfate sulfurtransferase, TST

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Validation Data Gallery

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  WB analysis of liver tissue using 16311-1-AP

  WB result of anti-TST (16311-1-AP) in Liver by Dr. Seow Chong Lee.

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  WB analysis using 16311-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16311-1-AP (TST antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 16311-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16311-1-AP (TST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16311-1-AP

  IP result of anti-TST (IP:16311-1-AP, 4ug; Detection:16311-1-AP 1:300) with HEK-293 cells lysate 2800ug.

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  IHC staining of human colon cancer using 16311-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16311-1-AP (TST antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 16311-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16311-1-AP (TST antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, liver tissue, human kidney tissue, HeLa cells, HepG2 cells, mouse kidney, rat kidney
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below

Product Information

16311-1-AP targets TST in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TST fusion protein Ag9346
• Full Name: thiosulfate sulfurtransferase (rhodanese)
• Calculated Molecular Weight: 297 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC010148
• Gene Symbol: TST
• Gene ID (NCBI): 7263
• RRID: AB_2211217
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thiosulfate sulfurtransferase (TST), also known as rhodanese, is a mitochondrial enzyme involved in cyanide detoxification and the formation of iron-sulfur proteins and the modification of sulfur-containing enzymes.

Publications

Species	Application	Title
human	WB	EMBO Mol Med; Coenzyme Q deficiency causes impairment of the sulfide oxidation pathway.; Authors - Marcello Ziosi
mouse	WB	EMBO Mol Med; CoQ deficiency causes disruption of mitochondrial sulfide oxidation, a new pathomechanism associated with this syndrome.; Authors - Marta Luna-Sánchez
mouse	WB	Antioxid Redox Signal; The Cysteine Dioxgenase Knockout Mouse: Altered Cysteine Metabolism in Nonhepatic Tissues Leads to Excess H2S/HS(-) Production and Evidence of Pancreatic and Lung Toxicity.; Authors - Roman Heather B HB
mouse,human	WB	Hum Mol Genet; Coenzyme Q10 modulates sulfide metabolism and links the mitochondrial respiratory chain to pathways associated to one carbon metabolism.; Authors - Pilar González-García
mouse	WB	Biochem Pharmacol; Cystathionine-γ-lyase (CSE) deficiency increases erythropoiesis and promotes mitochondrial electron transport via the upregulation of coproporphyrinogen III oxidase and consequent stimulation of heme biosynthesis.; Authors - Katalin Módis
mouse	WB	Amino Acids; Primary hepatocytes from mice lacking cysteine dioxygenase show increased cysteine concentrations and higher rates of metabolism of cysteine to hydrogen sulfide and thiosulfate.; Authors - Halina Jurkowska