TST Polyclonal antibody

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TST Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 16311-1-AP Copied!

Publications (11)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

EC:2.8.1.1, RDS, Rhodanese

• 

  WB analysis using 16311-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16311-1-AP (TST antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 16311-1-AP

  WB result of TST antibody (16311-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TST transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of liver tissue using 16311-1-AP

  WB result of anti-TST (16311-1-AP) in Liver by Dr. Seow Chong Lee.

• 

  WB analysis of human kidney using 16311-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16311-1-AP (TST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 16311-1-AP

  IP result of anti-TST (IP:16311-1-AP, 4ug; Detection:16311-1-AP 1:300) with HEK-293 cells lysate 2800ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 16311-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16311-1-AP (TST antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 16311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16311-1-AP (TST antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 16311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16311-1-AP (TST antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, human kidney tissue, liver tissue, HeLa cells, HepG2 cells, mouse kidney, rat kidney
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: mouse kidney tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16311-1-AP targets TST in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9346

  Product name: Recombinant human TST protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-297 aa of BC010148

  Sequence: MVHQVLYRALVSTKWLAESIRTGKLGPGLRVLDASWYSPGTREARKEYLERHVPGASFFDIEECRDTASPYEMMLPSEAGFAEYVGRLGISNHTHVVVYDGEHLGSFYAPRVWWMFRVFGHRTVSVLNGGFRNWLKEGHPVTSEPSRPEPAVFKATLDRSLLKTYEQVLENLESKRFQLVDSRSQGRFLGTEPEPDAVGLDSGHIRGAVNMPFMDFLTEDGFEKGPEELRALFQTKKVDLSQPLIATCRKGVTACHVALAAYLCGKPDVAVYDGSWSEWFRRAPPESRVSQGKSEKA

• Full Name: thiosulfate sulfurtransferase (rhodanese)
• Calculated Molecular Weight: 297 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC010148
• Gene Symbol: TST
• Gene ID (NCBI): 7263
• RRID: AB_2211217
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16762
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thiosulfate sulfurtransferase (TST), also known as rhodanese, is a mitochondrial enzyme involved in cyanide detoxification and the formation of iron-sulfur proteins and the modification of sulfur-containing enzymes.

Publications

What published studies show

Anti-TST (Thiosulfate Sulfurtransferase) polyclonal antibody, catalog #16311-1-AP, has 11 citations appearing in journals including Redox Biology, Pharmacological Research, Free Radical Biology and Medicine, EMBO Molecular Medicine, Antioxidants & Redox Signaling, Biochemical Pharmacology, Frontiers in Pharmacology, Amino Acids, and Human Molecular Genetics. Associated research fields include sulfur metabolism, mitochondrial sulfide oxidation, metabolic syndrome, vascular inflammation, colorectal cancer, cysteine homeostasis, and coenzyme Q deficiency.

Automatically generated from the 11 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Redox Biol; Cth/Mpst double ablation results in early onset fatty liver disease in lean mice; Authors - Antonia Katsouda
mouse	WB	Pharmacol Res; Protective role of 3-mercaptopyruvate sulfurtransferase (MPST) in the development of metabolic syndrome and vascular inflammation; Authors - Paraskevas Zampas
human	WB	Free Radic Biol Med; The role of 3-mercaptopyruvate sulfurtransferase in colorectal cancer progression and tumor angiogenesis.; Authors - Katie C Rouse
human	WB	EMBO Mol Med; Coenzyme Q deficiency causes impairment of the sulfide oxidation pathway.; Authors - Marcello Ziosi
mouse	WB	EMBO Mol Med; CoQ deficiency causes disruption of mitochondrial sulfide oxidation, a new pathomechanism associated with this syndrome.; Authors - Marta Luna-Sánchez
mouse	WB	Antioxid Redox Signal; The Cysteine Dioxgenase Knockout Mouse: Altered Cysteine Metabolism in Nonhepatic Tissues Leads to Excess H2S/HS(-) Production and Evidence of Pancreatic and Lung Toxicity.; Authors - Roman Heather B HB