Anticorps Monoclonal anti-CD206

• Phare
• Validé par KD/KO

CD206 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

113

CloneNo.

2A6A10

Avis (1)

N° de cat : 60143-1-Ig

Synonymes

CD206, CLEC13D, Macrophage mannose receptor 1, Mannose receptor, mannose receptor, C type 1, MMR, MRC1

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Galerie de données de validation

• 

  WB analysis of human placenta using 60143-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60143-1-Ig (CD206 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 60143-1-Ig

  human liver tissue were subjected to SDS PAGE followed by western blot with 60143-1-Ig (CD206 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 60143-1-Ig

  IP Result of anti-CD206 (IP:60143-1-Ig, 5ug; Detection:60143-1-Ig 1:300) with human placenta tissue lysate 1520ug.

• 

  IHC staining of human lung cancer using 60143-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60143-1-Ig (CD206 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60143-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60143-1-Ig (CD206 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 60143-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60143-1-Ig (CD206 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 60143-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60143-1-Ig (CD206 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human lung cancer using 60143-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CD206 antibody (60143-1-Ig, Clone: 2A6A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human lung cancer using 60143-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CD206 antibody (60143-1-Ig, Clone: 2A6A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, tissu hépatique humain
• Résultats positifs en IP: tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du poumon humain,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:10000-1:40000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 24 publications below
• IHC: See 43 publications below
• IF: See 65 publications below
• FC: See 4 publications below

Informations sur le produit

60143-1-Ig cible CD206 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mannose receptor, C type 1
• Masse moléculaire calculée: 166 kDa
• Poids moléculaire observé: 170 kDa
• Numéro d’acquisition GenBank: NM_002438
• Symbole du gène: MRC1
• Identification du gène (NCBI): 4360
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia. This antibody recognizes the intracellular carboxy-terminal part of CD206 and MRC1L1.

Publications

Species	Application	Title
	IF	ACS Nano; Tailoring Chemoimmunostimulant Bioscaffolds for Inhibiting Tumor Growth and Metastasis after Incomplete Microwave Ablation.; Authors - Yuting Shen
	IF	ACS Nano; Smart Liposomal Nanocarrier Enhanced the Treatment of Ischemic Stroke through Neutrophil Extracellular Traps and Cyclic Guanosine Monophosphate-Adenosine Monophosphate Synthase-Stimulator of Interferon Genes (cGAS-STING) Pathway Inhibition of Ischemic Penumbra; Authors - Shanbo Sun
human	IHC,IF	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
human	FC	Nat Commun; P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression.; Authors - Eilam Yeini
human	IHC	Nat Commun; Mitochondrial fission induces immunoescape in solid tumors through decreasing MHC-I surface expression.; Authors - Xinyuan Lei
pig	IF	Mater Today Bio; The spatial form periosteal-bone complex promotes bone regeneration by coordinating macrophage polarization and osteogenic-angiogenic events; Authors - C. Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kenzo (Verified Customer) (03-07-2023)

Works great with minimal background for immunofluorescence

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Optic nerve

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