CD11c/Integrin Alpha X Polyclonal antibody

CD11c/Integrin Alpha X Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

59

Cat no : 17342-1-AP

Synonyms

CD11c, CD11 antigen-like family member C, Leu M5, ITGAX, Integrin alpha-X

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Validation Data Gallery

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  WB analysis using 17342-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 17342-1-AP (CD11c/Integrin Alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 17342-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 17342-1-AP (ITGAX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 17342-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17342-1-AP (CD11c/Integrin Alpha X antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 17342-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17342-1-AP (CD11c/Integrin Alpha X antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 17342-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17342-1-AP (CD11c/Integrin Alpha X antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: THP-1 cells, human plasma
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 13 publications below
• IHC: See 26 publications below
• IF: See 17 publications below
• FC: See 4 publications below

Product Information

17342-1-AP targets CD11c/Integrin Alpha X in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD11c/Integrin Alpha X fusion protein Ag11380
• Full Name: integrin, alpha X (complement component 3 receptor 4 subunit)
• Calculated Molecular Weight: 1169 aa, 129 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC038237
• Gene Symbol: CD11c
• Gene ID (NCBI): 3687
• RRID: AB_2129787
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.

Publications

Species	Application	Title
human	IHC	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
	IHC	Acta Pharm Sin B; Celastrol targets adenylyl cyclase-associated protein 1 to reduce macrophages-mediated inflammation and ameliorates high fat diet-induced metabolic syndrome in mice.; Authors - Yuyu Zhu
	FC	Biomaterials; Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia.; Authors - Haoran Wang
human	IF	J Immunother Cancer; TSH-TSHR axis promotes tumor immune evasion.; Authors - Zhenghao Wu
	FC	Bioact Mater; Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.; Authors - Haoran Wang
	IF	Theranostics; Encapsulation of LXR ligand by D-Nap-GFFY hydrogel enhances anti-tumorigenic actions of LXR and removes LXR-induced lipogenesis.; Authors - Ke Feng

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