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CD209 Polyclonal antibody

CD209 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC,ELISA

Conjugate

Unconjugated

Cat no : 25404-1-AP

Synonyms

CD209, CD209 antigen, CD209 molecule, CDSIGN, CLEC4L, DC SIGN, DC SIGN1



Tested Applications

Positive WB detected inhuman placenta tissue
Positive FC detected inU-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25404-1-AP is CD209 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD209 fusion protein Ag21863
Full Name CD209 molecule
Calculated Molecular Weight 404 aa, 45.7 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC110615
Gene Symbol CD209
Gene ID (NCBI) 30835
RRIDAB_2880062
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD209 (DC-SIGN, CLEC4L) is a C-type lectin receptor that is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens, including bacteria, viruses, and parasites. CD209 is preferentially expressed on dendritic cells (DCs). It mediates transient adhesion of DCs with T cells. It has been reported that CD209 plays a role in capture and transmission of HIV from DC to T cells (PMID: 10721995).

Protocols

Product Specific Protocols
WB protocol for CD209 antibody 25404-1-APDownload protocol
FC protocol for CD209 antibody 25404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Immunity

SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells through C-type lectin receptors and Tweety family member 2.

Authors - Qiao Lu
  • KD Validated