CD209 Polyclonal antibody

• Featured Product
• KD/KO Validated

CD209 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 25404-1-AP

Synonyms

CD209, CD209 antigen, CD209 molecule, CDSIGN, CLEC4L, DC SIGN, DC SIGN1

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Validation Data Gallery

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  WB analysis of human placenta using 25404-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 25404-1-AP (CD209 Antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

The immunogen of 25404-1-AP is CD209 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD209 fusion protein Ag21863
• Full Name: CD209 molecule
• Calculated Molecular Weight: 404 aa, 45.7 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC110615
• Gene Symbol: CD209
• Gene ID (NCBI): 30835
• RRID: AB_2880062
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD209 (DC-SIGN, CLEC4L) is a C-type lectin receptor that is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens, including bacteria, viruses, and parasites. CD209 is preferentially expressed on dendritic cells (DCs). It mediates transient adhesion of DCs with T cells. It has been reported that CD209 plays a role in capture and transmission of HIV from DC to T cells (PMID: 10721995).

Publications

Species	Application	Title
human	WB	Immunity; SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells through C-type lectin receptors and Tweety family member 2.; Authors - Qiao Lu ; KD Validated
human	WB	Int J Mol Sci; Profiling of Early Immune Responses to Vaccination Using THP-1-Derived Dendritic Cells; Authors - Lei Ye
human	WB	J Neurooncol; Exploring the prognostic value of BRMS1 + microglia based on single-cell anoikis regulator patterns in the immunologic microenvironment of GBM; Authors - Songyun Zhao