CD11B/Integrin Alpha M Polyclonal antibody

CD11B/Integrin Alpha M Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20991-1-AP

Synonyms

CD11B, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6



Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20991-1-AP targets CD11B/Integrin Alpha M in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Calculated Molecular Weight 93 kDa
Observed Molecular Weight 110-170 kDa
GenBank Accession NumberNM_000632
Gene Symbol ITGAM
Gene ID (NCBI) 3684
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Protocols

Product Specific Protocols
IHC protocol for CD11B/Integrin Alpha M antibody 20991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

J Nanobiotechnology

Synergy of nanodiamond-doxorubicin conjugates and PD-L1 blockade effectively turns tumor-associated macrophages against tumor cells

Authors - Hua‑Zhen Xu
humanIHC

Cancer Lett

Piribedil disrupts the MLL1-WDR5 interaction and sensitizes MLL-rearranged acute myeloid leukemia (AML) to doxorubicin-induced apoptosis.

Authors - Xiong Zhang
humanWB

Cancer Immunol Immunother

Novel roles of VAT1 expression in the immunosuppressive action of diffuse gliomas.

Authors - Pei Yang
IF

J Nanobiotechnology

Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer.

Authors - Shen-Jun Yuan
IHC

Neuropharmacology

Trans-cinnamaldehyde improves memory impairment by blocking microglial activation through the destabilization of iNOS mRNA in mice challenged with lipopolysaccharide.

Authors - Liqing Zhang
WB,IHC

Mol Neurobiol

Myeloid TBK1 Deficiency Induces Motor Deficits and Axon Degeneration Through Inflammatory Cell Infiltration.

Authors - Weisong Duan