Anticorps Polyclonal de lapin anti-MMP7

• Phare
• Validé par KD/KO

MMP7 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

86

N° de cat : 10374-2-AP

Synonymes

MMP 7, Matrix metalloproteinase-7, Matrix metalloproteinase 7, Matrin, Matrilysin

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Galerie de données de validation

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  WB analysis using 10374-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10374-2-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 10374-2-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10374-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10374-2-AP (MMP7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10374-2-AP (MMP7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human pancreas cancer using 10374-2-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using 10374-2-AP (MMP7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human pancreas cancer using 10374-2-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using 10374-2-AP (MMP7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of PC-3 using 10374-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using MMP7 antibody (10374-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules COLO 320, cellules NIH/3T3, cellules PC-3, cellules SKOV-3, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer de la prostate humain, tissu de cancer de l'estomac humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du pancréas humain,
• Résultats positifs en IF/ICC: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 71 publications below
• IHC: See 18 publications below
• IF: See 1 publications below

Informations sur le produit

10374-2-AP cible MMP7 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MMP7 Protéine recombinante Ag0550
• Nom complet: matrix metallopeptidase 7 (matrilysin, uterine)
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC003635
• Symbole du gène: MMP7
• Identification du gène (NCBI): 4316
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Matrix metalloproteinase-7 (MMP-7)/ matrilysin is a member of the MMP family, but is structurally different from the other MMPs by virtue of the absence of a conserved COOH-terminal protein domain. MMPs are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and cancer metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP-7 degrades proteoglycans, fibronectin, elastin and casein, and is involved in wound healing, tumor progression, pulmonary fibrosis, and development of choroidal neovascularization in age-related macular degeneration. The expression of MMP-7 is increased in most tumors. This antibody can only recognize the full-length of MMP7.

Publications

Species	Application	Title
human	WB	Cancer Res; Hypoxic tumor-derived exosomal miR-301a mediates M2 macrophage polarization via PTEN/PI3Kγ to promote pancreatic cancer metastasis.; Authors - Xiaofeng Wang
human	WB	Cancer Res; Ubiquitin-like protein FAT10 promotes the invasion and metastasis of hepatocellular carcinoma by modifying β-catenin degradation.; Authors - Rongfa Yuan
human	IHC	Cancer Res; KRAS-NFκB-YY1-miR-489 signaling axis controls pancreatic cancer metastasis.; Authors - Peng Yuan
human	WB	Oncogene; Transducin (β)-like 1 X-linked receptor 1 promotes gastric cancer progression via the ERK1/2 pathway.; Authors - Q Zhou
human	WB	Clin Cancer Res; BATF2 Deficiency Promotes Progression in Human Colorectal Cancer via Activation of HGF/MET Signaling: A Potential Rationale for Combining MET Inhibitors with IFNs.; Authors - Zebing Liu
human,mouse	WB,IHC	Int J Cancer; Modification of α2,6-sialylation mediates the invasiveness and tumorigenicity of non-small cell lung cancer cells in vitro and in vivo via Notch1/Hes1/MMPs pathway.; Authors - Qingmin Yuan

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