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Brevican Polyclonal antibody

Brevican Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19017-1-AP

Synonyms

BCAN, UNQ2525/PRO6018, CSPG7, Brevican core protein, Brain-enriched hyaluronan-binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using Brevican Polyclonal antibody (19017-1-AP)

    WB analysis of human brain using 19017-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using Brevican Polyclonal antibody (19017-1-AP)

    WB analysis of SH-SY5Y using 19017-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using Brevican Polyclonal antibody (19017-1-AP)

    WB analysis of COLO 320 using 19017-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using Brevican Polyclonal antibody (19017-1-AP)

    WB analysis of Y79 using 19017-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using Brevican Polyclonal antibody (19017-1-AP)

    IHC staining of human cerebellum using 19017-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19017-1-AP (Brevican antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using Brevican Polyclonal antibody (19017-1-AP)

    IHC staining of human cerebellum using 19017-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19017-1-AP (Brevican antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Brevican Polyclonal antibody (19017-1-AP)

    IHC staining of mouse brain using 19017-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19017-1-AP (Brevican antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman brain tissue, COLO 320 cells, SH-SY5Y cells, Y79 cells
Positive IHC detected inhuman cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19017-1-AP targets Brevican in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Brevican fusion protein Ag13552
Full Name brevican
Calculated Molecular Weight 99 kDa
Observed Molecular Weight 145 kDa, 72kDa, 80 kDa
GenBank Accession NumberBC010571
Gene Symbol BCAN
Gene ID (NCBI) 63827
RRIDAB_10643526
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Brevican is a brain-specific proteoglycan belonging to the aggrecan family that share a number of structural features. Brevican contains a hyaluronic acid-binding domain in its N-terminus and a lectin-like domain in its C-terminus. Expression of brevican is highly specific in the brain and increases as the brain develops. It may play a role in maintaining the extracellular environment of mature brain as a major constituent of the adult brain extracellular matrix (PMID:9117262). Brevican core protein exists as a 145 kDa full-length form and a 80 kDa N terminally truncated form (PMID: 8144512). This antibody can recognize the 72 kDa isoform of target.

Protocols

Product Specific Protocols
WB protocol for Brevican antibody 19017-1-APDownload protocol
IHC protocol for Brevican antibody 19017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols