Anticorps Polyclonal de lapin anti-MLYCD

• Phare
• Validé par KD/KO

MLYCD Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

22

N° de cat : 15265-1-AP

Synonymes

malonyl CoA decarboxylase, MCD, MLYCD

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Galerie de données de validation

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  WB analysis of human testis using 15265-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15265-1-AP (MLYCD antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15265-1-AP

  IP result of anti-MLYCD (IP:15265-1-AP, 4ug; Detection:15265-1-AP 1:1000) with mouse liver tissue lysate 1840 ug.

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  IHC staining of human kidney using 15265-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15265-1-AP (MLYCD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15265-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15265-1-AP (MLYCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain,
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

15265-1-AP cible MLYCD dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MLYCD Protéine recombinante Ag7334
• Nom complet: malonyl-CoA decarboxylase
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC000286
• Symbole du gène: MLYCD
• Identification du gène (NCBI): 23417
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MLYCD, also named as MCD, is a 50-55 kDa protein. It is an enzyme that catalyzes the conversion of malonyl-CoA to acetyl-CoA. In the fatty acid biosynthesis MCD selectively removes malonyl-CoA and thus assures that methyl-malonyl-CoA is the only chain elongating substrate for fatty acid synthase and that fatty acids with multiple methyl side chains are produced. In peroxisomes it may be involved in degrading intraperoxisomal malonyl-CoA, which is generated by the peroxisomal beta-oxidation of odd chain-length dicarboxylic fatty acids. 66 kDa MLYCD protein has been identified in normal cell lines. Some patients give the 68-83 kDa smeary band. (PMID:12955715)

Publications

Species	Application	Title
human	WB	Hepatology; Acetyl-CoA carboxylase α promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients.; Authors - Ming-Da Wang
mouse	WB	Proc Natl Acad Sci U S A; Ribosomal protein-Mdm2-p53 pathway coordinates nutrient stress with lipid metabolism by regulating MCD and promoting fatty acid oxidation.; Authors - Yong Liu
mouse	WB	Cell Rep; Acetyl-CoA carboxylase 1 is a suppressor of the adipocyte thermogenic program; Authors - Adilson Guilherme ; KO Validated
		PLoS Pathog; Integrated systems biology analysis of KSHV latent infection reveals viral induction and reliance on peroxisome mediated lipid metabolism.; Authors - Zoi E Sychev
rat	WB	Int J Mol Sci; The Impact of Nicotine along with Oral Contraceptive Exposure on Brain Fatty Acid Metabolism in Female Rats; Authors - Shahil H Patel
mouse	WB	J Mol Cell Cardiol; C-Myc induced compensated cardiac hypertrophy increases free fatty acid utilization for the citric acid cycle.; Authors - Olson Aaron K AK

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