FASN Polyclonal antibody

• Featured Product
• KD/KO Validated

FASN Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

217

Reviews (2)

Cat no : 10624-2-AP

Synonyms

Type I fatty acid synthase, fatty acid synthase, FAS

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Validation Data Gallery

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  WB analysis using 10624-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10624-2-AP (FASN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10624-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10624-2-AP (FASN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10624-2-AP

  WB result of FASN antibody (10624-2-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FASN transfected HeLa cells.

• 

  IP experiment of mouse liver using 10624-2-AP

  IP result of anti-FASN (IP:10624-2-AP, 4ug; Detection:10624-2-AP 1:500) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human breast cancer using 10624-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 10624-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brown adipose using 10624-2-AP

  Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10624-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FASN antibody (10624-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  FC experiment of HeLa using 10624-2-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human FASN (10624-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, SH-SY5Y cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human breast cancer tissue, mouse brown adipose tissue, rat brown adipose tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 199 publications below
• IHC: See 30 publications below
• IF: See 14 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Product Information

10624-2-AP targets FASN in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, zebrafish, bovine, sheep, goat, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FASN fusion protein Ag0975
• Full Name: fatty acid synthase
• Calculated Molecular Weight: 272 kDa
• Observed Molecular Weight: 250-272 kDa
• GenBank Accession Number: BC007909
• Gene Symbol: FASN
• Gene ID (NCBI): 2194
• RRID: AB_2100801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.

Publications

Species	Application	Title
mouse	WB	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
mouse	WB	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu
mouse	WB	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang
mouse	WB	Nat Commun; Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity; Authors - Jinjie Duan
mouse	IF	Nat Commun; A nanoemulsion targeting adipose hypertrophy and hyperplasia shows anti-obesity efficiency in female mice; Authors - Yichao Lu
mouse	WB	Sci Transl Med; A small molecule targeting ALOX12-ACC1 ameliorates nonalcoholic steatohepatitis in mice and macaques.; Authors - Xiao-Jing Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

K (Verified Customer) (12-20-2020)

I got good results with this Ab for WB at (1:1000) and IHC (1:200)

• Applications: Western Blot, Immunohistochemistry
• Cell Tissue Type: mouse liver tissues and rat liver

FH

Iram (Verified Customer) (09-18-2020)

FASN antibody gives a very clean bands.Excellent antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 for western
• Cell Tissue Type: Human colon cancer

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