Anticorps Polyclonal de lapin anti-MIF

• Phare
• Validé par KD/KO

MIF Polyclonal Antibody for WB, IP, IF, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

16

Avis (5)

N° de cat : 20415-1-AP

Synonymes

GIF, GLIF, L dopachrome isomerase, L dopachrome tautomerase, MIF, MMIF, Phenylpyruvate tautomerase

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Galerie de données de validation

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  WB analysis using 20415-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20415-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20415-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20415-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 20415-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 20415-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 20415-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 20415-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 20415-1-AP

  IP result of anti-MIF (IP:20415-1-AP, 4ug; Detection:20415-1-AP 1:1000) with mouse spleen tissue lysate 4000ug.

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  IF Staining of U-251 using 20415-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using MIF antibody (20415-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of THP-1 using 20415-1-AP

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human MIF (20415-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293T, cellules HL-60, cellules HUVEC, cellules Jurkat, cellules MG U-87, cellules U-937, cellules Y79, tissu splénique de rat
• Résultats positifs en IP: tissu splénique de souris
• Résultats positifs en IF/ICC: cellules U-251,
• Résultats positifs en FC (Intra): cellules THP-1
• Résultats positifs en cytométrie: cellules THP-1

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 11 publications below
• IF: See 5 publications below

Informations sur le produit

20415-1-AP cible MIF dans les applications de WB, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MIF Protéine recombinante Ag14058
• Nom complet: macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• Masse moléculaire calculée: 115 aa, 12 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC000447
• Symbole du gène: MIF
• Identification du gène (NCBI): 4282
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MIF is a pleiotropic cytokine that contributes to the pathogenesis of many autoimmune diseases through its upstream immunoregulatory function and its polymorphic genetic locus. MIF is a highly conserved protein of 12.5 kDa, with evolutionarily ancient homologues in plants, protozoans, nematodes, and invertebrates.

Publications

Species	Application	Title
human	IF	Stem Cell Res Ther; DPSCs regulate epithelial-T cell interactions in oral submucous fibrosis; Authors - S Y Wang
human	WB	Cancer Lett; Macrophage migration inhibitory factor promotes tumor aggressiveness of esophageal squamous cell carcinoma via activation of Akt and inactivation of GSK3β.; Authors - Rui-Min Liu ; KD Validated
human	WB	Cell Prolif; TSP50 promotes hepatocyte proliferation and tumour formation by activating glucose-6-phosphate dehydrogenase (G6PD).; Authors - Xiaojun Zhang
mouse	WB	Front Endocrinol (Lausanne); Exercise prevents fatal stress-induced myocardial injury in obese mice; Authors - Yaoshan Dun
mouse	IF	Food Chem Toxicol; The crosstalk between M1 macrophage polarization and energy metabolism disorder contributes to polystyrene nanoplastics-triggered testicular inflammation; Authors - Siwen Li
human	WB	Mol Carcinog; Macrophage migration inhibitory factor promotes the invasion and metastasis of oral squamous cell carcinoma through matrix metalloprotein-2/9.; Authors - Sha-Sha Wang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tianyi (Verified Customer) (01-12-2024)

Antigen retrieval in TE buffer, incubated with AF594 for 2 h at RT

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Ovarian tissue

MIF Antibody Immunofluorescence validation (1:200 dilution) in Ovarian tissue (Cat no:20415-1-AP)

FH

sarah (Verified Customer) (10-27-2023)

antibody worked great for WB

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T, HCT116

FH

Sarah (Verified Customer) (01-24-2023)

This antibody worked well despite low protein concentration.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HCT116

FH

Emma (Verified Customer) (03-15-2022)

Nice antibody have used overnight @ 1:1000 on cell lysates and conditioned media. Produces a band at the correct size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: PC3

FH

Ryan (Verified Customer) (02-27-2019)

Tissue was fixed in PFA with no additional antigen retrieval. Co-localisation with microglia based on known markers (not shown).

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish brain (Microglia) at 3dpf

MIF Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain (Microglia) at 3dpf (Cat no:20415-1-AP)

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