Anticorps Polyclonal de lapin anti-TAU

TAU Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications

40

Avis (3)

N° de cat : 10274-1-AP

Synonymes

DDPAC, FTDP 17, MAPT, MAPTL, MSTD, MTBT1, MTBT2, Neurofibrillary tangle protein, Paired helical filament tau, PHF tau, PPND, TAU

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Galerie de données de validation

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  WB analysis of mouse brain using 10274-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10274-1-AP (TAU antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 10274-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10274-1-AP (TAU Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 10274-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10274-1-AP (TAU Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human gliomas using 10274-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10274-1-AP (TAU antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 10274-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10274-1-AP (TAU antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse brain using 10274-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TAU antibody (10274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of mouse brain using 10274-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TAU antibody (10274-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cerveau de rat
• Résultats positifs en IHC: tissu cérébral humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-Fro: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 30 publications below
• IHC: See 3 publications below
• IF: See 9 publications below

Informations sur le produit

10274-1-AP cible TAU dans les applications de WB, IHC, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TAU Protéine recombinante Ag0354
• Nom complet: microtubule-associated protein tau
• Masse moléculaire calculée: 37-46, 79-81 kDa
• Poids moléculaire observé: 45-70 kDa, 100 kDa
• Numéro d’acquisition GenBank: BC000558
• Symbole du gène: TAU
• Identification du gène (NCBI): 4137
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (8752131,15965697). Present polyclonal anti-Tau antibody was produced by immunizing animals with N-terminal of Tau and can detect approx 45-70 kDa and 100-kDa bands in brain tissues.

Publications

Species	Application	Title
mouse	WB	Sci Adv; Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease.; Authors - Xinwen Zhang
human		Brain; Tau accelerates α-synuclein aggregation and spreading in Parkinson's disease.; Authors - Lina Pan
mouse	IHC	Hum Mol Genet; Parkin deletion causes cerebral and systemic amyloidosis in human mutated tau over-expressing mice.; Authors - Rodríguez-Navarro Jose A JA
rat	WB	Acta Neuropathol Commun; Laterality and region-specific tau phosphorylation correlate with PTSD-related behavioral traits in rats exposed to repetitive low-level blast.; Authors - Georgina Perez Garcia
mouse,rat	WB	Free Radic Biol Med; The neuroprotective effects of phosphocreatine on amyloid beta 25-35-induced differentiated neuronal cell death through inhibition of AKT/GSK-3β /Tau/APP/CDK5 pathways in vivo and vitro; Authors - Jie Ai
mouse	IF	Mol Ther Nucleic Acids; Implications of miR-148a-3p/p35/PTEN signaling in tau hyperphosphorylation and autoregulatory feedforward of Akt/CREB in Alzheimer's disease.; Authors - Li Zeng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

manohar (Verified Customer) (03-06-2024)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Neuronal stem cells

FH

David (Verified Customer) (01-02-2024)

Produces a single band at around 45kDa which is concordant with the data sheet.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y lysate

FH

Tanusree (Verified Customer) (02-14-2019)

The antibody worked well with the mouse primary cells and tissue.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse brain

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