TDP-43 (human specific) Monoclonal antibody

TDP-43 (human specific) Monoclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1


human and More (1)







Cat no : 60019-2-Ig


ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, TDP-43 (human specific)

Tested Applications

Positive WB detected inMCF-7 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman gliomas tissue, human pancreas cancer tissue, human brain(FTLD-U) tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Sample-dependent, check data in validation data gallery

Product Information

60019-2-Ig targets TDP-43 (human specific) in WB, IP, IHC, FC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, yeast cell
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen fusion protein
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC001487
Gene symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 60019-2-Ig is a mouse monoclonal antibody recognizing the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Notably this antibody only recognizes human TDP-43 but not reacts with mouse or rat TDP-43.


Product Specific Protocols
WB protocol for TDP-43 (human specific) antibody 60019-2-IgDownload protocol
IHC protocol for TDP-43 (human specific) antibody 60019-2-IgDownload protocol
IP protocol for TDP-43 (human specific) antibody 60019-2-IgDownload protocol
FC protocol for TDP-43 (human specific) antibody 60019-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Neurosci

Leukocyte Derived Microvesicles as Disease Progression Biomarkers in Slow Progressing Amyotrophic Lateral Sclerosis Patients.

Authors - Daisy Sproviero

Dis Model Mech

Amyotrophic lateral sclerosis mutant TDP-43 may cause synaptic dysfunction through altered dendritic spine function.

Authors - Tongcui Jiang

Cell Mol Life Sci

Phosphorylated and aggregated TDP-43 with seeding properties are induced upon mutant Huntingtin (mHtt) polyglutamine expression in human cellular models.

Authors - Laurent Coudert

Front Aging Neurosci

Early Cognitive/Social Deficits and Late Motor Phenotype in Conditional Wild-Type TDP-43 Transgenic Mice.

Authors - Julio A Alfieri

Neuropathol Appl Neurobiol

The role of lysosomes and autophagosomes in Frontotemporal Lobar Degeneration.

Authors - Hamish Dc Bain

Acta Neuropathol Commun

RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases.

Authors - Mahlon Collins


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Lidiya (Verified Customer) (05-26-2022)

The antibody works great! Antibody was used for WB in Neuroblastoma cell lines (SK-N-BE(2)C and IMR32) with HRP-conjugated secondary. Antibody was also used for a Proximity Ligation Assay to assess interaction with another protein in SK-N-BE(2)C cells. 1:50 of antibody was used for this assay.

  • Applications: Western Blot, Other
  • Primary Antibody Dilution: 1:2000 (WB)
  • Cell Tissue Type: Neuroblastoma Cell Lines
TDP-43 (human specific) Antibody Western Blot,Other validation (1:2000 (WB) dilution) in Neuroblastoma Cell Lines (Cat no:60019-2-Ig)

David (Verified Customer) (01-13-2020)

Great antibody for several applications. Works nicely for immunoblot and immunofluourescence. Can also be used for RNA immunoprecipitation.

  • Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
  • Primary Antibody Dilution: 1:500 (IF); 1:1000 (WB); 30µg (RIP)
  • Cell Tissue Type: SH-SY5Y