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TDP-43 (human specific) Monoclonal antibody
TDP-43 (human specific) Monoclonal Antibody for FC, IHC, IP, WB, ELISA
Cat no : 60019-2-Ig
Validation Data Gallery
|Positive WB detected in||LNCaP cells, rabbit brain tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human gliomas tissue, human pancreas cancer tissue, human brain(FTLD-U) tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:5000-1:20000|
|Sample-dependent, check data in validation data gallery|
60019-2-Ig targets TDP-43 (human specific) in WB, IP, IHC, FC, CoIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, yeast cell|
|Host / Isotype||Mouse / IgG1|
|Full Name||TAR DNA binding protein|
|Calculated molecular weight||43 kDa|
|Observed molecular weight||43 kDa|
|GenBank accession number||BC001487|
|Gene ID (NCBI)||23435|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 60019-2-Ig is a mouse monoclonal antibody recognizing the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Notably this antibody only recognizes human TDP-43 but not reacts with mouse or rat TDP-43.
|Product Specific Protocols|
|WB protocol for TDP-43 (human specific) antibody 60019-2-Ig||Download protocol|
|IHC protocol for TDP-43 (human specific) antibody 60019-2-Ig||Download protocol|
|IP protocol for TDP-43 (human specific) antibody 60019-2-Ig||Download protocol|
|FC protocol for TDP-43 (human specific) antibody 60019-2-Ig||Download protocol|
|Click here to view our Standard Protocols|
Amyloid fibrils in disease FTLD-TDP are composed of TMEM106B not TDP-43.
A single-cell atlas of human and mouse white adipose tissue.
Mutations in UBQLN2 cause dominant X-linked juvenile and adult-onset ALS and ALS/dementia.
The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity.
TREM2 interacts with TDP-43 and mediates microglial neuroprotection against TDP-43-related neurodegeneration.
TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Lidiya (Verified Customer) (05-26-2022)
The antibody works great! Antibody was used for WB in Neuroblastoma cell lines (SK-N-BE(2)C and IMR32) with HRP-conjugated secondary. Antibody was also used for a Proximity Ligation Assay to assess interaction with another protein in SK-N-BE(2)C cells. 1:50 of antibody was used for this assay.
David (Verified Customer) (01-13-2020)
Great antibody for several applications. Works nicely for immunoblot and immunofluourescence. Can also be used for RNA immunoprecipitation.