Anticorps Polyclonal de lapin anti-MAG

MAG Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

17

Avis (1)

N° de cat : 14386-1-AP

Synonymes

GMA, MAG, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A

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Galerie de données de validation

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  WB analysis using 14386-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14386-1-AP (MAG antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 14386-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14386-1-AP (MAG Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse spinal cord using 14386-1-AP

  mouse spinal cord tissue were subjected to SDS PAGE followed by western blot with 14386-1-AP (MAG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 14386-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14386-1-AP (MAG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14386-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14386-1-AP (MAG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 14386-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14386-1-AP (MAG antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14386-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14386-1-AP (MAG antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de moelle épinière de souris, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below
• IF: See 5 publications below

Informations sur le produit

14386-1-AP cible MAG dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAG Protéine recombinante Ag5845
• Nom complet: myelin associated glycoprotein
• Masse moléculaire calculée: 69 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC053347
• Symbole du gène: MAG
• Identification du gène (NCBI): 4099
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.

Publications

Species	Application	Title
human	WB	Brain; A role of the frontotemporal lobar degeneration risk factor TMEM106B in myelination.; Authors - Tuancheng Feng
mouse	WB	Acta Neuropathol Commun; TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss.; Authors - Tuancheng Feng
mouse	IF, WB	J Cereb Blood Flow Metab; Connexin43 promotes angiogenesis through activating the HIF-1α/VEGF signaling pathway under chronic cerebral hypoperfusion.; Authors - Weiwei Yu
mouse	WB	Mol Neurobiol; Quercetin Alleviates Demyelination Through Regulating Microglial Phenotype Transformation to Mitigate Neuropsychiatric Symptoms in Mice with Vascular Dementia.; Authors - Zihu Tan
mouse	IF	Neurosci Bull; Digoxin Ameliorates Glymphatic Transport and Cognitive Impairment in a Mouse Model of Chronic Cerebral Hypoperfusion; Authors - Jie Cao
rat	WB	CNS Neurosci Ther; Proteomic analysis of the effects of caffeine in a neonatal rat model of hypoxic-ischemic white matter damage.; Authors - Liu Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emma (Verified Customer) (02-22-2022)

Works well on FFPE prostate tissue by IF. (Tris-EDTA antigen retrieval).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate

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