LINGO1 Polyclonal antibody

LINGO1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 19097-1-AP

Synonyms

LERN1, LINGO1, LRRN6A, UNQ201

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Validation Data Gallery

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  WB analysis of mouse brain using 19097-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19097-1-AP (LINGO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19097-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19097-1-AP (LINGO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 19097-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 19097-1-AP (LINGO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19097-1-AP targets LINGO1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LINGO1 fusion protein Ag13553
• Full Name: leucine rich repeat and Ig domain containing 1
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC011057
• Gene Symbol: LINGO1
• Gene ID (NCBI): 84894
• RRID: AB_10638484
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LINGO-1 is a transmembrane receptor that contains 12 leucine-rich repeats and an immunoglobulin domain. It is expressed primarily in the central nervous system. LINGO-1 binds Nogo-66 receptor (NgR) and p75 and that is a functional component of the NgR/p75 signaling complex (PMID: 14966521). This protein has a calculated molecular weight of 70 kDa and migrates at higher molecular weight due to glycosylation (PMID: 21872576; 25257685; 18326721).