OMG Polyclonal antibody

OMG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12701-1-AP

Synonyms

OMG, OMGP

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Validation Data Gallery

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  WB analysis of mouse spinal cord using 12701-1-AP

  mouse spinal cord tissue were subjected to SDS PAGE followed by western blot with 12701-1-AP (OMG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12701-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12701-1-AP (OMG Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 12701-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12701-1-AP (OMG Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 12701-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12701-1-AP (OMG Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse spinal cord tissue, mouse brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12701-1-AP targets OMG in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OMG fusion protein Ag3210
• Full Name: oligodendrocyte myelin glycoprotein
• Calculated Molecular Weight: 440 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC018050
• Gene Symbol: OMG
• Gene ID (NCBI): 4974
• RRID: AB_2877877
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OMG (oligodendrocyte myelin glycoprotein), also known as OMGP. It is expected to be located in cell membrane, the protein is mainly expressed in the central nervous system in both neurons and oligodendrocytes. The calculated molecular weight of OMG is 50 kDa, and o-glycosylated in its Ser/Thr-rich repeat domain. It is also a cell adhesion molecule contributing to the interactive process required for myelination in the central nervous system. Axonal regeneration in the adult spinal cord is thought to be at least partially blocked after injury by inhibitory myelin components, including Nogo, myelin associated glycoprotein (MAG) and oligodendrocyte-myelin glycoprotein (OMGP).

Publications

Species	Application	Title
rat	WB	Brain Res; Electroacupuncture promotes axonal regrowth by attenuating the myelin-associated inhibitors-induced RhoA/ROCK pathway in cerebral ischemia/reperfusion rats.; Authors - Suhui Chen
human	WB	Viruses; Circulating Plasma Exosomal Proteins of Either SHIV-Infected Rhesus Macaque or HIV-Infected Patient Indicates a Link to Neuropathogenesis; Authors - Partha K Chandra