Anticorps Polyclonal de lapin anti-MACF1

• Phare
• Validé par KD/KO

MACF1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 13058-1-AP

Synonymes

620 kDa actin binding protein, ABP620, ACF7, KIAA0465, KIAA1251, MACF, MACF1, Macrophin 1, OFC4, Trabeculin alpha

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Galerie de données de validation

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  WB analysis using 13058-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13058-1-AP (MACF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13058-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13058-1-AP (MACF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 13058-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13058-1-AP (MACF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 13058-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13058-1-AP (MACF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 13058-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13058-1-AP (MACF1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 13058-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13058-1-AP (MACF1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 13058-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13058-1-AP (MACF1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 13058-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13058-1-AP (MACF1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of U-251 using 13058-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using MACF1 antibody (13058-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of NIH/3T3 using 13058-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using MACF1 antibody (13058-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MG U-87, cellules NIH/3T3, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu pulmonaire humain, tissu cardiaque humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U-251, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below

Informations sur le produit

13058-1-AP cible MACF1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MACF1 Protéine recombinante Ag3730
• Nom complet: microtubule-actin crosslinking factor 1
• Masse moléculaire calculée: 5430 aa, 620 kDa
• Poids moléculaire observé: 600 kDa
• Numéro d’acquisition GenBank: BC007330
• Symbole du gène: MACF1
• Identification du gène (NCBI): 23499
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MACF1, also called ACF7 (actin crosslinking family 7), is a member of the spectraplakin family and is a ubiquitously expressed 614 kDa protein. This protein facilitates actin-microtubule interactions at the cell periphery and couples the microtubule network to cellular junctions. It displays a conserved role in membrane protrusion formation during evolution. The two major MACF1 isoforms characterized to date, MACF1a and MACF1b, result from alternative splicing. Both proteins contain N-terminal actin-binding domains (ABD), followed by plakin domains, extended spectrin repeat domains, EF-hand motifs, and C-terminal microtubule-binding domains (MTBDs). MACF1 is expressed in numerous tissue, including the skin, brain, skeletal muscle, and heart.

Publications

Species	Application	Title
human	IHC	Gastroenterology; Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors; Authors - Mingjun Sun
human	WB	Nat Commun; The genomic landscape of cholangiocarcinoma reveals the disruption of post-transcriptional modifiers.; Authors - Yaodong Zhang
mouse	WB	Cell Death Differ; MACF1 promotes osteoblast differentiation by sequestering repressors in cytoplasm.; Authors - Lifang Hu ; KD Validated
mouse	WB	PLoS Genet; BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.; Authors - Ying Hsu
human	WB	Oncotarget; LRP6 promotes invasion and metastasis of colorectal cancer through cytoskeleton dynamics.; Authors - Qian Yao
human	IHC	Biomed Pharmacother; Proteomics-based identification of tumor relevant proteins in lung adenocarcinoma.; Authors - Xin Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joleen (Verified Customer) (06-03-2019)

MACF1 should be the top band but there were a lot of non specific bands as well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

MACF1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:13058-1-AP)

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