CLASP1 Polyclonal antibody

CLASP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 20803-1-AP

Synonyms

CLASP1, CLIP associating protein 1, hOrbit1, KIAA0622, MAST1, Multiple asters homolog 1, Protein Orbit homolog 1

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Validation Data Gallery

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  WB analysis using 20803-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20803-1-AP (CLASP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20803-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20803-1-AP (CLASP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20803-1-AP

  IP result of anti-CLASP1 (IP:20803-1-AP, 4ug; Detection:20803-1-AP 1:2000) with HeLa cells lysate 1750 ug.

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  IHC staining of human testis using 20803-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20803-1-AP (CLASP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 20803-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20803-1-AP (CLASP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, rat brain tissue, SH-SY5Y cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20803-1-AP targets CLASP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLASP1 fusion protein Ag14724
• Full Name: cytoplasmic linker associated protein 1
• Calculated Molecular Weight: 1538 aa, 169 kDa
• Observed Molecular Weight: 162 kDa
• GenBank Accession Number: BC112940
• Gene Symbol: CLASP1
• Gene ID (NCBI): 23332
• RRID: AB_10697808
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLASP1 is a member of CLASP family. Members of the CLASP family of proteins are involved in multiple MT-dependent processes, including Golgi complex organization and trafficking, kinetochore fiber dynamics and flux, mitotic spindle assembly, and the stabilization of MT plus ends near the cortex. CLASP proteins regulate MT dynamics by promoting MT rescue and suppressing MT catastrophe. Recent work showed that CLASP1 activity is required for proper positioning of the mitotic spindle in both the C. elegans embryo and mammalian cells. Recent work has also identified roles CLASP1 in the regulation of spindle positioning.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sammy (Verified Customer) (11-05-2023)

Excellent antibody for detection of CLASP1 via Western Blotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HUVEC

CLASP1 Antibody Western Blot validation (1:1000 dilution) in HUVEC (Cat no:20803-1-AP)