Alpha Actin Polyclonal antibody
Alpha Actin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 23660-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | rat skeletal muscle tissue, mouse skeletal muscle tissue |
Positive IP detected in | mouse skeletal muscle tissue |
Positive IHC detected in | human colon tissue, human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | C2C12 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 57 publications below |
IHC | See 1 publications below |
IF | See 12 publications below |
FC | See 1 publications below |
Product Information
The immunogen of 23660-1-AP is Alpha Actin Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Alpha Actin fusion protein Ag19151 |
Full Name | actin, alpha 1, skeletal muscle |
Calculated molecular weight | 42 kDa |
Observed molecular weight | 40-42 kDa |
GenBank accession number | BC012597 |
Gene symbol | ACTA1 |
Gene ID (NCBI) | 58 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen Affinity purified |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
IHC protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
IF protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
IP protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
FC protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Redox Biol Mitochondrial dysfunction by TFAM depletion disrupts self-renewal and lineage differentiation of human PSCs by affecting cell proliferation and YAP response. | ||
J Hazard Mater Chlorine disinfection elevates the toxicity of polystyrene microplastics to human cells by inducing mitochondria-dependent apoptosis. | ||
Free Radic Biol Med SIRT3 promotes metabolic maturation of human iPSC-derived cardiomyocytes via OPA1-controlled mitochondrial dynamics | ||
Oncogene CFIm25-regulated lncRNA acv3UTR promotes gastric tumorigenesis via miR-590-5p/YAP1 axis. | ||
Nanotoxicology Evaluation of toxicity of halloysite nanotubes and multi-walled carbon nanotubes to endothelial cells in vitro and blood vessels in vivo. | ||