Anticorps Polyclonal de lapin anti-TIP47

• Phare
• Validé par KD/KO

TIP47 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA, IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

35

Avis (1)

N° de cat : 10694-1-AP

Synonymes

M6PRBP1, Perilipin 3, Perilipin-3, Mannose-6-phosphate receptor-binding protein 1, 47 kDa MPR-binding protein

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Galerie de données de validation

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  WB analysis of 3T3-L1 using 10694-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10694-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10694-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10694-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10694-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10694-1-AP

  IP result of anti-TIP47 (IP:10694-1-AP, 3ug; Detection:10694-1-AP 1:1000) with HeLa cells lysate 1000ug.

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  IHC staining of mouse liver using 10694-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10694-1-AP (TIP47 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 10694-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 10694-1-AP (TIP47 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 10694-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 10694-1-AP (TIP47 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MEF cells using 10694-1-AP

  IF result of anti-TIP47 (10694-1-AP,1:500) with MEF cell by Dr.Hector Alex Saka.

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  IF Staining of HeLa using 10694-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using TIP47 antibody (10694-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin was stained with CL594-phalloidin.

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  IF Staining of HeLa using 10694-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using TIP47 antibody (10694-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 10694-1-AP

  1X10^6 HeLa cells were stained with 0.2ug TIP47 antibody (10694-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules 3T3-L1, cellules HeLa, cellules Jurkat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu hépatique de souris, tissu ovarien de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MEF, cellules HeLa traitées à l'acide oléique
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 28 publications below
• IHC: See 4 publications below
• IF: See 8 publications below

Informations sur le produit

10694-1-AP cible TIP47 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TIP47 Protéine recombinante Ag1028
• Nom complet: mannose-6-phosphate receptor binding protein 1
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC007566
• Symbole du gène: TIP47/PLIN3
• Identification du gène (NCBI): 10226
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mannose 6-phosphate receptors (M6PRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. M6PRBP1 (mannose-6-phosphate receptor binding protein 1), also known as TIP47, PLIN3 or PP17, interacts with the cytoplasmic domains of both cation-independent and cation-dependent M6PRs, and is required for endosome-to-Golgi transport. In addition to M6PR recycling, M6PRBP1 plays a role in lipid droplet biogenesis, and is also implicated in rhodopsin photobleaching and viral infection. M6PRBP1 has been found to be expressed in a variety of human tissues (including colon, liver and lung parenchyme, mammary gland, and skin) and is overexpressed in certain cancer cell lines. It binds to lipid droplets and also occurs in cytosol and on endosomal membranes.

Publications

Species	Application	Title
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
mouse	WB	Nat Commun; Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.; Authors - Fangjun Yu
human	IF	Nat Commun; The autophagy protein ATG9A enables lipid mobilization from lipid droplets.; Authors - Elodie Mailler
human	WB	Hepatology; CIDEC/Fsp27 is regulated by PPARα and plays a critical role in fasting- and diet-induced hepatosteatosis.; Authors - Cédric Langhi
mouse	WB	Nat Commun; O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity.; Authors - Yunfan Yang
human	IF	J Cell Biol; A role for ubiquitin ligases and Spartin/SPG20 in lipid droplet turnover.; Authors - Eastman Scott W SW

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christine (Verified Customer) (09-19-2023)

Tested on HEK293T cells stimulated by 300 uM oleic acid overnight to stimulate the formation of lipid droplets. This antibody (tested only at 1:100 so far) decorates the lipid droplets, it worked well.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HEK293T

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