IGF2R-Specific Polyclonal antibody

IGF2R-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

17

Reviews (2)

Cat no : 20253-1-AP

Synonyms

CD222, CI Man 6 P receptor, CI MPR, CIMPR, IGF II receptor, IGF2R, IGF2R-Specific, M6P R, M6P/IGF2 receptor, M6P/IGF2R, M6PR, MPR 300, MPR1, MPRI

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Validation Data Gallery

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  WB analysis using 20253-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20253-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 20253-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20253-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 20253-1-AP

  IP result of anti-IGF2R-Specific (IP:20253-1-AP, 4ug; Detection:20253-1-AP 1:500) with HepG2 cells lysate 3480ug.

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  IHC staining of human liver cancer using 20253-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 20253-1-AP (IGF2R-Specific Antibody) at dilution of 1:50.

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  IHC staining of human liver cancer using 20253-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 20253-1-AP (IGF2R-Specific Antibody) at dilution of 1:50.

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  IHC staining of human liver using 20253-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 20253-1-AP (IGF2R-Specific Antibody) at dilution of 1:50.

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  IHC staining of human liver using 20253-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 20253-1-AP (IGF2R-Specific Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 20253-1-AP

  Immunofluorescent analysis of HepG2 cells using 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 20253-1-AP

  1X10^6 HepG2 cells were stained with .2ug IGF2R-Specific antibody (20253-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Tested Applications

• Positive WB detected in: A431 cells, L02 cells, HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

20253-1-AP targets IGF2R-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: insulin-like growth factor 2 receptor
• Calculated Molecular Weight: 274 kDa
• Observed Molecular Weight: 300 kDa
• GenBank Accession Number: NM_000876
• Gene Symbol: IGF2R
• Gene ID (NCBI): 3482
• RRID: AB_10859779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGF2R, also named as MPRI, CI Man-6-P receptor, CI-MPR, M6PR, IGF-II receptor, M6P/IGF2R, MPR 300 and CD222, belongs to the MRL1/IGF2R family. IGF2R transports of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. IGF2R is a lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. IGF2R acts as a positive regulator of T-cell coactivation, by binding DPP4. The antibody is specific to IGF2R.

Publications

Species	Application	Title
human	WB	Hum Mol Genet; Progranulin regulates lysosomal function and biogenesis through acidification of lysosomes.; Authors - Yoshinori Tanaka
rat	WB	J Agric Food Chem; Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.; Authors - Shu Yang
human	WB, IF	Mol Biol Cell; Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.; Authors - Stephen C Ireland
human	WB	J Cancer; Trop2 binding IGF2R induces gefitinib resistance in NSCLC by remodeling the tumor microenvironment.; Authors - Xia Sun
human	WB	Life Sci Alliance; Identification and characterization of a membrane receptor that binds to human STC1.; Authors - Hin Ting Wan
rat	WB,IHC	J Sci Food Agric; Grandmaternal betaine supplementation enhances hepatic IGF2 expression in F2 rat offspring through modification of promoter DNA methylation.; Authors - Shu Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tiphaine (Verified Customer) (06-20-2022)

Localization seems to be coherent

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: HeLa cells

IGF2R-Specific Antibody Immunofluorescence validation (1/100 dilution) in HeLa cells (Cat no:20253-1-AP)

FH

Q (Verified Customer) (03-22-2021)

A reliable antibody to detect endogenous IGF2R with WB. Although there are a few non specific bands at lower levels.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain

IGF2R-Specific Antibody Western Blot validation (1:2000 dilution) in mouse brain (Cat no:20253-1-AP)