IGF2R-Specific Polyclonal antibody
IGF2R-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 20253-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, L02 cells, HepG2 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 14 publications below |
IHC | See 2 publications below |
IF | See 4 publications below |
Product Information
20253-1-AP targets IGF2R-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, bovine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | insulin-like growth factor 2 receptor |
Calculated molecular weight | 274 kDa |
Observed molecular weight | 300 kDa |
GenBank accession number | NM_000876 |
Gene symbol | IGF2R |
Gene ID (NCBI) | 3482 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IGF2R, also named as MPRI, CI Man-6-P receptor, CI-MPR, M6PR, IGF-II receptor, M6P/IGF2R, MPR 300 and CD222, belongs to the MRL1/IGF2R family. IGF2R transports of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. IGF2R is a lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. IGF2R acts as a positive regulator of T-cell coactivation, by binding DPP4. The antibody is specific to IGF2R.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
IHC protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
IF protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
IP protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
FC protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Hum Mol Genet Progranulin regulates lysosomal function and biogenesis through acidification of lysosomes. | ||
J Agric Food Chem Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams. | ||
Mol Biol Cell Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking. | ||
J Cancer Trop2 binding IGF2R induces gefitinib resistance in NSCLC by remodeling the tumor microenvironment. | ||
Life Sci Alliance Identification and characterization of a membrane receptor that binds to human STC1. | ||
J Sci Food Agric Grandmaternal betaine supplementation enhances hepatic IGF2 expression in F2 rat offspring through modification of promoter DNA methylation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tiphaine (Verified Customer) (06-20-2022) | Localization seems to be coherent
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FH Q (Verified Customer) (03-22-2021) | A reliable antibody to detect endogenous IGF2R with WB. Although there are a few non specific bands at lower levels.
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