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ADRP/Perilipin-2 Polyclonal antibody

ADRP/Perilipin 2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15294-1-AP

Synonyms

ADFP, Adipophilin, ADRP, ADRP/Perilipin 2, Perilipin 2, PLIN2



Tested Applications

Positive WB detected inmouse liver tissue, K-562 cells, rat liver tissue, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue, human liver tissue, human renal cell carcinoma tissue, human prostate cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inoleic acid treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15294-1-AP targets ADRP/Perilipin 2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, zebrafish, pig, canine, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADRP/Perilipin 2 fusion protein Ag7539
Full Name adipose differentiation-related protein
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC005127
Gene Symbol ADFP
Gene ID (NCBI) 123
RRIDAB_2878122
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.

Protocols

Product Specific Protocols
WB protocol for ADRP/Perilipin 2 antibody 15294-1-APDownload protocol
IHC protocol for ADRP/Perilipin 2 antibody 15294-1-APDownload protocol
IF protocol for ADRP/Perilipin 2 antibody 15294-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nature

iPS-cell-derived microglia promote brain organoid maturation via cholesterol transfer

Authors - Dong Shin Park
humanIF

Nature

The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle.

Authors - Simona Ricciardi
mouse,humanIF,IHC

Cell Metab

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Authors - Yi Fu
mouseIF

Nat Commun

Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagy

Authors - Donghai Cui
mouseWB

Mol Cell

Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation.

Authors - Anyuan He
mouseWB

Nat Commun

O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity.

Authors - Yunfan Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Liliana (Verified Customer) (02-13-2024)

WORKS VERY WELL ON PH9, 1:2000 AND ON PH6, 1:1000

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: SKIN WITH SEBACCEUS ADENOMA
ADRP/Perilipin 2 Antibody Immunohistochemistry validation (1:2000 dilution) in SKIN WITH SEBACCEUS ADENOMA (Cat no:15294-1-AP)
FH

Christine (Verified Customer) (09-19-2023)

Tried on HEK293T cells treated with 300 uM oleic acid overnight to induce the formation of lipid droplets. This antibody decorates some droplets but was a bit weak. TIP47 antibody (10694-1-AP) performed better (clearer detection of the droplets) at the same concentration (1:100).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: HEK293T
FH

Kamal (Verified Customer) (07-05-2023)

Worked well when diluted with 1X PBS.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse Liver tissue
FH

Christin (Verified Customer) (03-09-2023)

Great antibody for WB with low background signal.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human iPSC-derived microglia and macrophages
FH

Nick (Verified Customer) (04-20-2022)

Helped localize expression of Plin2

  • Applications: Western Blot, Immunoprecipitation, Cell culture
  • Cell Tissue Type: 3T3-L1
FH

Boyan (Verified Customer) (05-09-2019)

Very good for WB

  • Applications: Western Blot,
  • Cell Tissue Type: MEF