RAB9A-Specific Polyclonal antibody

RAB9A-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18719-1-AP

Synonyms

RAB9, RAB9A, RAB9A-Specific, Ras related protein Rab 9A

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Validation Data Gallery

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  WB analysis of HEK-293 using 18719-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18719-1-AP (RAB9A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18719-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18719-1-AP (RAB9A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 18719-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18719-1-AP (RAB9A-Specific Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18719-1-AP targets RAB9A-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: RAB9A, member RAS oncogene family
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC017265
• Gene Symbol: RAB9A
• Gene ID (NCBI): 9367
• RRID: AB_2175604
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB9A, also named as RAB9, is a member of the Ras superfamily of small Rab GTPases. It is located in late endosomes. Rab9A interacts with its effector proteins p40 and TIP47 to promote the MPR (mannose 6-phosphate receptor)-associated lysosomal enzyme transport between late endosomes and the trans Golgi network. The antibody is specific to RAB9A.