Anticorps Monoclonal anti-Lamin B1

• Phare
• Validé par KD/KO

Lamin B1 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

300

CloneNo.

3C10G12

Avis (7)

N° de cat : 66095-1-Ig

Synonymes

ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  WB analysis of multi-cells/tissue using 66095-1-Ig

  Western blot on multiple cells/tissues with anti-LMNB1 (66095-1-Ig) at dilution 1:20000.

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  WB analysis using 66095-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66095-1-Ig (Lamin B1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66095-1-Ig

  WB result of Lamin B1 antibody (66095-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin B1 transfected HeLa cells.

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  IP experiment of HeLa using 66095-1-Ig

  IP Result of anti-Lamin B1 (IP:66095-1-Ig, 4ug; Detection:66095-1-Ig 1:20000) with HeLa cells lysate 3560ug.

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  IHC staining of human pancreas cancer using 66095-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66095-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66095-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66095-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse eye using 66095-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using Lamin B1 antibody (66095-1-Ig, Clone: 3C10G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 66095-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 66095-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Lamin B1 (66095-1-Ig, Clone:3C10G12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules NCI-H1299, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules PC-12, multi-cellules/tissus
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa, tissu oculaire de souris
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:250-1:1000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 269 publications below
• IHC: See 2 publications below
• IF: See 16 publications below
• IP: See 4 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

66095-1-Ig cible Lamin B1 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, Lapin, poisson-zèbre, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lamin B1 Protéine recombinante Ag20522
• Nom complet: lamin B1
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 66-70 kDa
• Numéro d’acquisition GenBank: BC012295
• Symbole du gène: LMNB1
• Identification du gène (NCBI): 4001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.

Publications

Species	Application	Title
mouse	IF	Nat Cell Biol; Ceramide-rich microdomains facilitate nuclear envelope budding for non-conventional exosome formation.; Authors - Subhash B Arya
		J Hepatol; OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.; Authors - Weiqi Dai
		Cell Rep Med; Management of prostate cancer by targeting 3βHSD1 after enzalutamide and abiraterone treatment.; Authors - Zejie Mei
human	WB	Nat Commun; ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling; Authors - Maximilian Hirschenberger
human	WB	J Clin Invest; FAM117B promotes gastric cancer growth and drug resistance by targeting the KEAP1/NRF2 signaling pathway; Authors - Yunjiang Zhou
human	WB	Cell Host Microbe; Structural Basis for a Species-Specific Determinant of an SIV Vif Protein toward Hominid APOBEC3G Antagonism.; Authors - Jennifer M Binning

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

S (Verified Customer) (12-12-2022)

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: LUNG Fibroblast

Lamin B1 Antibody Western Blot validation (1000 dilution) in LUNG Fibroblast (Cat no:66095-1-Ig)

FH

WEI (Verified Customer) (03-08-2022)

Specfic bands around predicted size with higher non-specific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart

FH

P. (Verified Customer) (05-15-2021)

Good antibody!

• Applications: Western Blot
• Cell Tissue Type: H9C2

Lamin B1 Antibody Western Blot validation ( dilution) in H9C2 (Cat no:66095-1-Ig)

FH

Eiko (Verified Customer) (11-18-2020)

Used RIPA buffer for western sample preparation, and 5% Skim milk TBS-T was used for blocking and antibody dilution.Since I could see clear lamin B1 signal, I am happy to use this antibody as one of my internal controls.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 (can be more diluted)
• Cell Tissue Type: hTERT-RPE1

FH

Tom (Verified Customer) (10-09-2020)

I observed a discrete ~70kDa Lamin B1 band using this antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Yuan (Verified Customer) (12-13-2019)

Did staining for human A549 cell.The Lamin B antibody had high background in nucleus. Please see attached image.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Human A549 cell

Lamin B1 Antibody Immunofluorescence, validation (1:250 dilution) in Human A549 cell (Cat no:66095-1-Ig)

FH

Shubham (Verified Customer) (03-14-2019)

good

• Applications: Western Blot,
• Cell Tissue Type: AC16

Lamin B1 Antibody Western Blot, validation ( dilution) in AC16 (Cat no:66095-1-Ig)

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