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Anticorps Polyclonal de lapin anti-LGALS3BP

LGALS3BP Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 23307-1-AP

Synonymes

90K, BTBD17B, Galectin 3 binding protein, LGALS3BP, M2BP, Mac 2 binding protein, MAC 2 BP, MAC2BP, Tumor associated antigen 90K

Galerie de données de validation

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  • Western Blot (WB) analysis of HepG2 cells using LGALS3BP Polyclonal antibody (23307-1-AP)

    WB analysis of HepG2 using 23307-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23307-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using LGALS3BP Polyclonal antibody (23307-1-AP)

    WB analysis of mouse brain using 23307-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 23307-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using LGALS3BP Polyclonal antibody (23307-1-AP)

    IHC staining of human breast cancer using 23307-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23307-1-AP (LGALS3BP Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using LGALS3BP Polyclonal antibody (23307-1-AP)

    IHC staining of human breast cancer using 23307-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23307-1-AP (LGALS3BP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using LGALS3BP Polyclonal antibody (23307-1-AP)

    IHC staining of human skin using 23307-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin slide using 23307-1-AP (LGALS3BP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LGALS3BP Polyclonal antibody (23307-1-AP)

    IF Staining of HepG2 using 23307-1-AP

    Immunofluorescent analysis of HepG2 cells using 23307-1-AP (LGALS3BP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Applications testées

Résultats positifs en WBcellules HepG2, tissu cérébral de souris
Résultats positifs en IHCtissu de cancer du sein humain, tissu cutané humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HepG2

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochimie (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

23307-1-AP cible LGALS3BP dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène LGALS3BP Protéine recombinante Ag19700
Nom complet lectin, galactoside-binding, soluble, 3 binding protein
Masse moléculaire calculée 585 aa, 65 kDa
Poids moléculaire observé 65-70 kDa
Numéro d’acquisition GenBankBC002403
Symbole du gène LGALS3BP
Identification du gène (NCBI) 3959
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ).

Protocole

Product Specific Protocols
WB protocol for LGALS3BP antibody 23307-1-APDownload protocol
IHC protocol for LGALS3BP antibody 23307-1-APDownload protocol
IF protocol for LGALS3BP antibody 23307-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols