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Galectin-1 Monoclonal antibody

Galectin-1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1









Cat no : 60223-1-Ig


14 kDa laminin binding protein, 14 kDa lectin, Gal 1, GAL1, Galaptin, Galectin 1, Galectin-1, HBL, HLBP14, HPL, Lactose binding lectin 1, LGALS1, S Lac lectin 1

Tested Applications

Positive WB detected inHCT 116 cells, human placenta tissue, human heart tissue, HepG2 cells, U2OS cells, A549 cells, HeLa cells, L02 cells
Positive IHC detected inhuman colon cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells, Saos-2 cells, human colon cancer tissue

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60223-1-Ig targets Galectin-1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Galectin-1 fusion protein Ag2432
Full Name lectin, galactoside-binding, soluble, 1
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC020675
Gene Symbol LGALS1
Gene ID (NCBI) 3956
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).


Product Specific Protocols
WB protocol for Galectin-1 antibody 60223-1-IgDownload protocol
IHC protocol for Galectin-1 antibody 60223-1-IgDownload protocol
IF protocol for Galectin-1 antibody 60223-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy

Authors - Kui Wang


Design of a peptidic inhibitor that targets the dimer interface of a prototypic galectin.

Authors - Maria Claudia Vladoiu

Sci Rep

Tissue and plasma levels of galectins in patients with high grade serous ovarian carcinoma as new predictive biomarkers.

Authors - Marilyne Labrie

Int J Oncol

Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer

Authors - Qi Zhang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Guorong (Verified Customer) (05-23-2022)

It is excellent with ARPE-19 cells but gives no signal with 293T

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: 293T and ARPE-19
Galectin-1 Antibody Western Blot validation (1:2000 dilution) in 293T and ARPE-19 (Cat no:60223-1-Ig)

Sushmita (Verified Customer) (12-16-2019)

This Galactin-1 antibody is working very fine which I applied to probe in western blot in cancer cell line.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:4000
  • Cell Tissue Type: Pancreatic cancer cell line
Galectin-1 Antibody Western Blot, validation (1:4000 dilution) in Pancreatic cancer cell line (Cat no:60223-1-Ig)