Galectin-1 Monoclonal antibody

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• KD/KO Validated

Galectin-1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

3G10D2

Reviews (2)

Cat no : 60223-1-Ig

Synonyms

Gal 1, Beta-galactoside-binding lectin L-14-I, 3G10D2, 14 kDa lectin, 14 kDa laminin-binding protein

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Validation Data Gallery

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  WB analysis using 60223-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60223-1-Ig (Galectin-1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HepG2 using 60223-1-Ig

  WB result of Galectin-1 antibody (60223-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin-1 transfected HepG2 cells.

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  WB analysis using 60223-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60223-1-Ig (Galectin-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 60223-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60223-1-Ig (Galectin-1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 60223-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 60223-1-Ig (GAL1,LGALS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 60223-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60223-1-Ig (Galectin-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60223-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60223-1-Ig (Galectin-1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 60223-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60223-1-Ig (Galectin-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 60223-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60223-1-Ig (Galectin-1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using 60223-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-1 antibody (60223-1-Ig, Clone: 3G10D2 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of Saos-2 using 60223-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using Galectin-1 antibody (60223-1-Ig, Clone: 3G10D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of U2OS using 60223-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Galectin-1 antibody (60223-1-Ig, Clone: 3G10D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HCT 116 cells, human placenta tissue, human heart tissue, HepG2 cells, U2OS cells, A549 cells, HeLa cells, L02 cells
• Positive IHC detected in: human colon cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human colon cancer tissue
• Positive IF/ICC detected in: U2OS cells, Saos-2 cells, human colon cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

60223-1-Ig targets Galectin-1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Galectin-1 fusion protein Ag2432
• Full Name: lectin, galactoside-binding, soluble, 1
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC020675
• Gene Symbol: Galectin-1
• Gene ID (NCBI): 3956
• RRID: AB_11182608
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).

Publications

Species	Application	Title
human		Autophagy; Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy; Authors - Kui Wang
human	WB	Oncotarget; Design of a peptidic inhibitor that targets the dimer interface of a prototypic galectin.; Authors - Maria Claudia Vladoiu
human	IF	Sci Rep; Tissue and plasma levels of galectins in patients with high grade serous ovarian carcinoma as new predictive biomarkers.; Authors - Marilyne Labrie
human	IF	Int J Oncol; Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer; Authors - Qi Zhang
human	WB	mBio; Herpes simplex virus type 1 modifies the protein composition of extracellular vesicles to promote neurite outgrowth and neuroinfection; Authors - Guorong Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guorong (Verified Customer) (05-23-2022)

It is excellent with ARPE-19 cells but gives no signal with 293T

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: 293T and ARPE-19

Galectin-1 Antibody Western Blot validation (1:2000 dilution) in 293T and ARPE-19 (Cat no:60223-1-Ig)

FH

Sushmita (Verified Customer) (12-16-2019)

This Galactin-1 antibody is working very fine which I applied to probe in western blot in cancer cell line.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: Pancreatic cancer cell line

Galectin-1 Antibody Western Blot, validation (1:4000 dilution) in Pancreatic cancer cell line (Cat no:60223-1-Ig)

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