Anticorps Polyclonal de lapin anti-JNK

JNK Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

N° de cat : 51151-1-AP

Synonymes

c Jun N terminal kinase 1, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1



Applications testées

Résultats positifs en WBcellules 4T1, cellules A431, cellules C6, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules Neuro-2a, cellules NIH/3T3, tissu cérébral de souris
Résultats positifs en IHCtissu de cancer du côlon humain, tissu de cancer du poumon humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IFcellules SH-SY5Y

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

51151-1-AP cible JNK dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéerat, Humain, porc, poulet, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet mitogen-activated protein kinase 8
Masse moléculaire calculée 48 kDa
Poids moléculaire observé 44-48 kDa, 50-55 kDa
Numéro d’acquisition GenBankNM_138982
Symbole du gène MAPK8
Identification du gène (NCBI) 5599
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease
  • JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.  

Protocole

Product Specific Protocols
WB protocol for JNK antibody 51151-1-APDownload protocol
IHC protocol for JNK antibody 51151-1-APDownload protocol
IF protocol for JNK antibody 51151-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Res

Mlkl knockout mice demonstrate the indispensable role of Mlkl in necroptosis.

Authors - Wu Jianfeng J
mouseWB

J Crohns Colitis

Mincle/Syk signaling promotes intestinal mucosal inflammation through induction of macrophage pyroptosis in Crohn's disease.

Authors - Wenbin Gong
mouseWB

Br J Pharmacol

Amentoflavone attenuates Listeria monocytogenes pathogenicity through an LLO-dependent mechanism.

Authors - Wang Tingting
mouseWB

J Transl Med

Angiotensin-(1-7) ameliorates sepsis-induced cardiomyopathy by alleviating inflammatory response and mitochondrial damage through the NF-κB and MAPK pathways

Authors - Xin-Sen Chen
humanWB

Biochim Biophys Acta Mol Basis Dis

Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages

Authors - Ling Zhou
humanWB

Aging (Albany NY)

Transplanting Rac1-silenced bone marrow mesenchymal stem cells promote neurological function recovery in TBI mice.

Authors - Dongdong Huang