Anticorps Polyclonal de lapin anti-JNK
JNK Polyclonal Antibody for IF, IHC, WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, souris et plus (3)
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugaison
Non conjugué
120
N° de cat : 51151-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules 4T1, cellules A431, cellules C6, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules Neuro-2a, cellules NIH/3T3, tissu cérébral de souris |
Résultats positifs en IHC | tissu de cancer du côlon humain, tissu de cancer du poumon humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF | cellules SH-SY5Y |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochimie (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 115 publications below |
IHC | See 2 publications below |
IP | See 2 publications below |
CoIP | See 1 publications below |
Informations sur le produit
51151-1-AP cible JNK dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris
Réactivité | Humain, souris |
Réactivité citée | rat, Humain, porc, poulet, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | Peptide |
Nom complet | mitogen-activated protein kinase 8 |
Masse moléculaire calculée | 48 kDa |
Poids moléculaire observé | 44-48 kDa, 50-55 kDa |
Numéro d’acquisition GenBank | NM_138982 |
Symbole du gène | MAPK8 |
Identification du gène (NCBI) | 5599 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Function
JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.
Tissue specificity
JNK1 is ubiquitously expressed.
Involvement in disease
JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.
Isoforms
There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.
Post-translational modifications
JNK1 is phosphorylated by MAPK kinases (MAPKK).
Cellular localization
JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.
Protocole
Product Specific Protocols | |
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WB protocol for JNK antibody 51151-1-AP | Download protocol |
IHC protocol for JNK antibody 51151-1-AP | Download protocol |
IF protocol for JNK antibody 51151-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Crohns Colitis Mincle/Syk signaling promotes intestinal mucosal inflammation through induction of macrophage pyroptosis in Crohn's disease. | ||
Br J Pharmacol Amentoflavone attenuates Listeria monocytogenes pathogenicity through an LLO-dependent mechanism. | ||
J Transl Med Angiotensin-(1-7) ameliorates sepsis-induced cardiomyopathy by alleviating inflammatory response and mitochondrial damage through the NF-κB and MAPK pathways | ||
Biochim Biophys Acta Mol Basis Dis Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages | ||
Aging (Albany NY) Transplanting Rac1-silenced bone marrow mesenchymal stem cells promote neurological function recovery in TBI mice. |