Anticorps Polyclonal de lapin anti-ISG15

• Phare
• Validé par KD/KO

ISG15 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications

54

Avis (2)

N° de cat : 15981-1-AP

Synonymes

IP17, Interferon-induced 17 kDa protein, Interferon-induced 15 kDa protein, IFI15, hUCRP

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Galerie de données de validation

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  WB analysis using 15981-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15981-1-AP (ISG15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15981-1-AP

  Untreated and IFN alpha treated A459 cells, untreated and IFN alpha treated HepG2 cells were subjected to SDS PAGE followed by western blot with 15981-1-AP (ISG15 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15981-1-AP

  WB result of ISG15 antibody (15981-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ISG15 transfected MCF-7 cells.

• 

  WB analysis of human brain using 15981-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15981-1-AP (ISG15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15981-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15981-1-AP (ISG15 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15981-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15981-1-AP (ISG15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 15981-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15981-1-AP (ISG15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15981-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15981-1-AP (ISG15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 15981-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15981-1-AP (ISG15 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 15981-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15981-1-AP (ISG15 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of mouse brain using 15981-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ISG15 antibody (15981-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 15981-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ISG15 antibody (15981-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 15981-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15981-1-AP (ISG15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HepG2, cellules MCF-7, cellules PC-3, tissu cérébral humain
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 46 publications below
• IHC: See 9 publications below
• IF: See 6 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

15981-1-AP cible ISG15 dans les applications de WB, IHC, IF/ICC, IF-P, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Chèvre, Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ISG15 Protéine recombinante Ag8782
• Nom complet: ISG15 ubiquitin-like modifier
• Masse moléculaire calculée: 165 aa, 18 kDa
• Poids moléculaire observé: 15-17 kDa
• Numéro d’acquisition GenBank: BC009507
• Symbole du gène: ISG15
• Identification du gène (NCBI): 9636
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA) and -beta (IFNB). ISG15 forms covalent conjugates with its target proteins in a process called ISGylation, which in mammals is known to play a role in antiviral immunity. ISG15 proteins possess two ubiquitin-like (UBL) domains and a highly conserved C-terminal LRGG sequence, the latter being known as the ubiquitin conjugation motif. Intracellular ISG15 are conjugated, via the LRGG motif, to target proteins through a process called ISGylation, which resembles largely ubiquitination, the process of formation of ubiquitin conjugates. Unconjugated extracellular ISG15, which are released from several types of human and murine cells, are known to possess cytokine-like activity.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng
human,mouse	IP,IHC,WB	Exp Mol Med; SIRT1 ISGylation accelerates tumor progression by unleashing SIRT1 from the inactive state to promote its deacetylase activity; Authors - Ji An Kang
human	IF	Nat Commun; Single cell RNA sequencing of human microglia uncovers a subset associated with Alzheimer's disease.; Authors - Marta Olah
human	WB	Nat Commun; ISGylation controls exosome secretion by promoting lysosomal degradation of MVB proteins.; Authors - Carolina Villarroya-Beltri
human	WB,IHC,IF,CoIP	J Exp Clin Cancer Res; ISG15 and ISGylation modulates cancer stem cell-like characteristics in promoting tumor growth of anaplastic thyroid carcinoma; Authors - Tong Xu ; KD Validated
human	WB	Cancer Lett; Induction of IFIT1/IFIT3 and inhibition of Bcl-2 orchestrate the treatment of myeloma and leukemia via pyroptosis; Authors - Yuanming He

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tinne (Verified Customer) (06-27-2023)

This antibody worked really well for detecting an up regulation of ISG15 in IF. Highly recommend!

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human hippocampal progenitor cells

FH

Chaohui (Verified Customer) (10-19-2021)

The antibody shows a high quality of free ISG15 and conjugated ISG15 spots

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293, THP1

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