EIF4E2 Polyclonal antibody

EIF4E2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, RIP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12227-1-AP

Synonyms

4E LP, 4EHP, eIF 4E type 2, eIF4E like protein 4E LP, eIF4E type 2, EIF4E2, EIF4EL3, IF4e, mRNA cap binding protein 4EHP



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, mouse kidney tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12227-1-AP targets EIF4E2 in WB, IP, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF4E2 fusion protein Ag2864
Full Name eukaryotic translation initiation factor 4E family member 2
Calculated Molecular Weight 245 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC021690
Gene Symbol EIF4E2
Gene ID (NCBI) 9470
RRIDAB_10642945
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EIF4E2 antibody 12227-1-APDownload protocol
IHC protocol for EIF4E2 antibody 12227-1-APDownload protocol
IP protocol for EIF4E2 antibody 12227-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

Authors - Ji Geng
humanWB,RIP

Cell Death Dis

α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation

Authors - Hao Wang
humanWB

iScience

The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex.

Authors - Limei Zou
humanWB,IHC

Eur J Med Res

N7-methylguanosin regulators-mediated methylation modification patterns and characterization of the immune microenvironment in lower-grade glioma

Authors - Aierpati Maimaiti
humanWB

Nat Struct Mol Biol

Isoform-resolved mRNA profiling of ribosome load defines interplay of HIF and mTOR dysregulation in kidney cancer

Authors - Yoichiro Sugimoto