USP18 Polyclonal antibody

USP18 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12153-1-AP

Synonyms

43 kDa ISG15 specific protease, hUBP43, ISG43, Ubl thiolesterase 18, UBP43, USP18

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Validation Data Gallery

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  WB analysis of MCF-7 using 12153-1-AP

  WB result of USP18 antibody(12153-1-AP) with MCF-7 cells (Control and CRISPR USP18).

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Tested Applications

• Positive WB detected in: MCF-7

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

12153-1-AP targets USP18 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP18 fusion protein Ag2785
• Full Name: ubiquitin specific peptidase 18
• Calculated Molecular Weight: 372 aa, 43 kDa
• Observed Molecular Weight: 43 kDa, 70 kDa
• GenBank Accession Number: BC014896
• Gene Symbol: USP18
• Gene ID (NCBI): 11274
• RRID: AB_2877830
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP18(UBP43) is a 43 kDa protein that is known as an ISG15-deconjugating enzyme and an inhibitor of type I IFN signaling independent of its enzyme activity. USP18 can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. It can be detected two bands(apparent MW of 38 and 35 kDa), the faster migrating one results from proteolytic processing in human primary hepatocytes(PMID:21779393). Sometimes the 70 kDa band is showed on the SDS-PAGE that it is considered as the complex of ISG15 and USP18(PMID: 14673145).

Publications

Species	Application	Title
human,mouse	WB,IHC	iScience; MiR-4769-3p suppresses adipogenesis in systemic sclerosis by negatively regulating the USP18/VDAC2 pathway; Authors - Bingsi Tang
human	WB	Exp Cell Res; USP18 promotes the proliferation, invasion, and migration of head and neck squamous cell carcinoma by deubiquitinating PLK1; Authors - Liang Geng
human	WB	Cancer Cell Int; Adipose stem cell exosomes, stimulated by pro-inflammatory factors, enhance immune evasion in triple-negative breast cancer by modulating the HDAC6/STAT3/PD-L1 pathway through the transporter UCHL1; Authors - Qin Zhu