USP18 Polyclonal antibody
USP18 Polyclonal Antibody for WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB,ELISA
Conjugate
Unconjugated
Cat no : 12153-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
12153-1-AP targets USP18 in WB,ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | USP18 fusion protein Ag2785 |
Full Name | ubiquitin specific peptidase 18 |
Calculated Molecular Weight | 372 aa, 43 kDa |
Observed Molecular Weight | 43 kDa, 70 kDa |
GenBank Accession Number | BC014896 |
Gene Symbol | USP18 |
Gene ID (NCBI) | 11274 |
RRID | AB_2877830 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
USP18(UBP43) is a 43 kDa protein that is known as an ISG15-deconjugating enzyme and an inhibitor of type I IFN signaling independent of its enzyme activity. USP18 can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. It can be detected two bands(apparent MW of 38 and 35 kDa), the faster migrating one results from proteolytic processing in human primary hepatocytes(PMID:21779393). Sometimes the 70 kDa band is showed on the SDS-PAGE that it is considered as the complex of ISG15 and USP18(PMID: 14673145).