Anticorps Polyclonal de lapin anti-INVS

• Phare
• Validé par KD/KO

INVS Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, FC, IHC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 10585-1-AP

Synonymes

INV, inversin, INVS, KIAA0573, Nephrocystin 2

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Galerie de données de validation

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  WB analysis of MCF-7 using 10585-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10585-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10585-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10585-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10585-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10585-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10585-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 10585-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10585-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10585-1-AP (INVS antibody) at dilution of 1:100 (under 25x lens).

• 

  IHC staining of human breast cancer using 10585-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10585-1-AP (INVS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 10585-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10585-1-AP (INVS antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 10585-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10585-1-AP (INVS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 10585-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10585-1-AP (INVS antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of hTERT-RPE1 cells using 10585-1-AP

  IF result from Dr. Corbit, Kevin. anti-INVS (also named as NPHP2; 10585-1-AP) marks the ‘inversin compartment’ and basal bodies of Human hTERT-RPE1 cells.

• 

  IF Staining of HeLa using 10585-1-AP

  Immunofluorescent analysis of Hela cells, using INVS antibody 10585-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HepG2, cellules Jurkat, cellules K-562, cellules L02, cellules Raji, cellules SH-SY5Y, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cirrhose hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules hTERT-RPE1

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• FC: See 1 publications below

Informations sur le produit

10585-1-AP cible INVS dans les applications de WB, IF, FC, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: INVS Protéine recombinante Ag0934
• Nom complet: inversin
• Masse moléculaire calculée: 118 kDa, 100 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC006370
• Symbole du gène: INVS
• Identification du gène (NCBI): 27130
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

INVS, short for inversin, plays a role in primary cilia function and involvement in the cell cycle. It is required for normal renal development and establishment of the left-right axis. INVS may be involved in Wnt signaling pathways by acting as a molecular switch between different them. Inhibits the canonical Wnt pathway by targeting cytoplasmic disheveled (DVL1) for degradation by the ubiquitin-proteasome. This suggests that it is required in renal development to oppose the repression of terminal differentiation of tubular epithelial cells by Wnt signaling. This antibody is a rabbit polyclonal antibody raised against part of the N-terminus of human INVS.

Publications

Species	Application	Title
mouse	WB,IF	EMBO J; Cell type-specific regulation of ciliary transition zone assembly in vertebrates.; Authors - Antonia Wiegering ; KO Validated
	IF	Proc Natl Acad Sci U S A; A complement factor H homolog, heparan sulfation, and syndecan maintain inversin compartment boundaries in C. elegans cilia.; Authors - Natalie Acker
rat	WB	Endocrinology; PCP protein inversin regulates testis function through changes in cytoskeletal organization of actin and microtubules.; Authors - Linxi Li ; KD Validated
rat	IHC	Virchows Arch; Afferent arteriolopathy and glomerular collapse but not segmental sclerosis induce tubular atrophy in old spontaneously hypertensive rats.; Authors - Leh Sabine S
human	IF	Am J Med Genet A; Early presentation of cystic kidneys in a family with a homozygous INVS mutation.; Authors - Machteld M Oud
human	FC	JCI Insight; Cilia proteins are biomarkers of altered flow in the vasculature.; Authors - Ankan Gupta

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