Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13559-1-AP targets APC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4427 Product name: Recombinant human ANAPC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 524-822 aa of BC032503 Sequence: ADRLLHQFSFSPEREIRNVELLKLRFGEAPMHFCEVMLKDMADSRRINANIREEDEKRPAEEQPPFGVYAVILSSEFWPPFKDEKLEVPEDIRAALEAYCKKYEQLKAMRTLSWKHTLGLVTMDVELADRTLSVAVTPVQAVILLYFQDQASWTLEELSKAVKMPVALLRRRMSVWLQQGVLREEPPGTFSVIEEERPQDRDNMVLIDSDDESDSGMASQADQKEEELLLFWTYIQAMLTNLESLSLDRIYNMLRMFVVTGPALAEIDLQELQGYLQKKVRDQQLVYSAGVYRLPKNCS Predict reactive species |
| Full Name | anaphase promoting complex subunit 2 |
| Calculated Molecular Weight | 822 aa, 94 kDa |
| Observed Molecular Weight | 97 kDa |
| GenBank Accession Number | BC032503 |
| Gene Symbol | APC2 |
| Gene ID (NCBI) | 29882 |
| RRID | AB_2227055 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UJX6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for APC2 antibody 13559-1-AP | Download protocol |
| IHC protocol for APC2 antibody 13559-1-AP | Download protocol |
| IP protocol for APC2 antibody 13559-1-AP | Download protocol |
| WB protocol for APC2 antibody 13559-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The APC2 antibody (Cat# 13559-1-AP) has 10 total citations published in journals including Nature Communications, Advanced Science, Cancer Letters, Oncogenesis, PLoS Pathogens, FASEB Journal, FEBS Journal, and bioRxiv. Research fields span cell cycle regulation, oocyte meiosis, DNA damage repair, pancreatic and cervical cancer, acute kidney injury, viral biology, agrin signaling, and Alzheimer's disease. Applications include WB, IF, and IHC in human and mouse models.
| Species | Application | Title |
|---|---|---|
Nat Commun CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I | ||
Nat Commun The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites. | ||
Adv Sci (Weinh) CCDC41 Drives Oocyte Meiotic Progression by Promoting Rab11a/Rab7-Positive Vesicle Fusion with Target Membranes. | ||
Adv Sci (Weinh) CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCC | ||
Cancer Lett UBE2C promotes pancreatic tumorigenesis by KRAS stabilization via APC/CCDH1-mediated WDR76 degradation | ||
Oncogenesis Human papillomavirus16 E7 enhances cell stemness by regulating the APC2/SPIN4/β-catenin axis in cervical cancer.
|
