|Positive WB detected in||mouse brain tissue, Y79 cells|
|Positive IHC detected in||human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||hTERT-RPE1 cells, MDCK cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
13812-1-AP targets NPHP4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, Canine samples.
|Tested Reactivity||human, mouse, Canine|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NPHP4 fusion protein Ag4875|
|Full Name||nephronophthisis 4|
|Calculated molecular weight||1426 aa, 158 kDa|
|Observed molecular weight||150 kDa|
|GenBank accession number||BC040520|
|Gene ID (NCBI)||261734|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
NPHP4, also named as KIAA0673, is a cilia-associated protein which involved in the organization of apical junctions in kidney cells together with NPHP1 and RPGRIP1L/NPHP8. It is necessary for normal photoreceptor ribbon synapse maintenance and outer segment formation, and for sperm development.
A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.
Nat Cell Biol
Lineage specificity of primary cilia in the mouse embryo.
Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.
Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.
J Cell Biol
The small GTPase RSG1 controls a final step in primary cilia initiation.
Primary Cilium Formation and Ciliary Protein Trafficking Is Regulated by the Atypical MAP Kinase MAPK15 in Caenorhabditis elegans and Human Cells.