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- KD/KO Validated
RPGRIP1L Polyclonal antibody
RPGRIP1L Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IP, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 55160-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, human kidney tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human pancreas tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
55160-1-AP targets RPGRIP1L in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, zebrafish, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | RPGRIP1-like |
| Calculated Molecular Weight | 151 kDa |
| GenBank Accession Number | NM_015272 |
| Gene Symbol | RPGRIP1L |
| Gene ID (NCBI) | 23322 |
| RRID | AB_10860269 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RPGRIP1L, also named as FTM, KIAA1005, belongs to the RPGRIP1 family. It negatively regulates signaling through the G-protein coupled thromboxane A2 receptor. RPGRIP1L may be involved in mechanisms like programmed cell death, craniofacial development, patterning of the limbs, and formation of the left-right axis.(PMID:17558409) Defects in RPGRIP1L are the cause of Joubert syndrome type 7 (JBTS7). Defects in RPGRIP1L are the cause of Meckel syndrome type 5 (MKS5). This antibody is specific to RPGRIP1L.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPGRIP1L antibody 55160-1-AP | Download protocol |
| IHC protocol for RPGRIP1L antibody 55160-1-AP | Download protocol |
| IP protocol for RPGRIP1L antibody 55160-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. | ||
Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. | ||
Sci Adv Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ. | ||
Am J Hum Genet Mutations in CSPP1, Encoding a Core Centrosomal Protein, Cause a Range of Ciliopathy Phenotypes in Humans. | ||
J Cell Biol TMEM231, mutated in orofaciodigital and Meckel syndromes, organizes the ciliary transition zone. | ||
Dev Cell Comparative Super-Resolution Mapping of Basal Feet Reveals a Modular but Distinct Architecture in Primary and Motile Cilia. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Alice (Verified Customer) (01-25-2019) | Cells fixed in MeOH at -20'C for 5mins.Blocked with 1% w/v Milk/PBSRPGRIP1L antibody diluted in block 1:1000 and co-stained with Acetylated alpha-Tubulin and gamma-tubulin.
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