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Anticorps Polyclonal de lapin anti-Histone H3

Histone H3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (9)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

N° de cat : 17168-1-AP

Synonymes

HIST2H3A, H3-clustered histone 15, H3-clustered histone 14, H3-clustered histone 13, H3C15


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  • Western Blot (WB) analysis of various lysates using Histone H3 Polyclonal antibody (17168-1-AP)

    WB analysis using 17168-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17168-1-AP (Histone H3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Histone-H3 Polyclonal antibody (17168-1-AP)

    WB analysis using 17168-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17168-1-AP (Histone-H3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using Histone-H3 Polyclonal antibody (17168-1-AP)

    WB analysis of mouse liver using 17168-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17168-1-AP (Histone-H3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Histone-H3 Polyclonal antibody (17168-1-AP)

    WB analysis of mouse brain using 17168-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17168-1-AP (Histone-H3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Histone H3 Polyclonal antibody (17168-1-AP)

    WB analysis of mouse skeletal muscle using 17168-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17168-1-AP (Histone-H3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using Histone H3 Polyclonal antibody (17168-1-AP)

    IP experiment of MCF-7 using 17168-1-AP

    IP result of anti-Histone-H3 (IP:17168-1-AP, 4ug; Detection:17168-1-AP 1:8000) with MCF-7 cells lysate 2120 ug.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Histone H3 Polyclonal antibody (17168-1-AP)

    IHC staining of human oesophagus cancer using 17168-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 17168-1-AP (Histone H3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Histone H3 Polyclonal antibody (17168-1-AP)

    IHC staining of human oesophagus cancer using 17168-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 17168-1-AP (Histone H3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Histone H3 Polyclonal antibody (17168-1-AP)

    IHC staining of human skin cancer using 17168-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17168-1-AP (Histone-H3 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Histone H3 Polyclonal antibody (17168-1-AP)

    IHC staining of human skin cancer using 17168-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17168-1-AP (Histone-H3 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Histone H3 Polyclonal antibody (17168-1-AP)

    IHC staining of human breast cancer using 17168-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17168-1-AP (Histone-H3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Histone H3 Polyclonal antibody (17168-1-AP)

    IHC staining of human breast cancer using 17168-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17168-1-AP (Histone-H3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Histone H3 Polyclonal antibody (17168-1-AP)

    IF Staining of HeLa using 17168-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Histone-H3 antibody (17168-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Histone H3 Polyclonal antibody (17168-1-AP)

    IF Staining of HeLa using 17168-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Histone-H3 antibody (17168-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Histone H3 Polyclonal antibody (17168-1-AP)

    IF Staining of HeLa using 17168-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Histone H3 antibody (17168-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Beta Actin antibody (66009-1-Ig, Clone: 2D4H5, red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Histone H3 Polyclonal antibody (17168-1-AP)

    IF Staining of HeLa using 17168-1-AP

    Immunofluorescent analysis of HeLa cells using 17168-1-AP (Histone-H3 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

"Histone H3 Antibodies" Comparison

View side-by-side comparison of Histone H3 antibodies from other vendors to find the one that best suits your research needs.

Applications testées

Résultats positifs en WBcellules HEK-293, cellules A549, cellules HeLa, cellules HepG, cellules MCF-7, cellules NIH/3T3, tissu cérébral de souris, tissu de muscle squelettique de souris, tissu hépatique de souris, tissu rénal de rat, tissu rénal de souris
Résultats positifs en IPcellules MCF-7,
Résultats positifs en IHCtissu de cancer de l'œsophage humain, tissu de cancer de la peau humain, tissu de cancer du sein humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HeLa,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

17168-1-AP cible Histone H3 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéebovin, canin, Chèvre, Humain, poulet, singe, arabidopsis, Grenouille, duck, fish
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Histone H3 Protéine recombinante Ag10644
Nom complet histone cluster 2, H3a
Masse moléculaire calculée 136 aa, 15 kDa
Poids moléculaire observé 15-17 kDa
Numéro d’acquisition GenBankBC015544
Symbole du gène Histone H3
Identification du gène (NCBI) 333932
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.

Protocole

Product Specific Protocols
WB protocol for Histone H3 antibody 17168-1-APDownload protocol
IHC protocol for Histone H3 antibody 17168-1-APDownload protocol
IF protocol for Histone H3 antibody 17168-1-APDownload protocol
IP protocol for Histone H3 antibody 17168-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

The molecular basis of tight nuclear tethering and inactivation of cGAS.

Authors - Baoyu Zhao
WB

Nature

A phase-separated nuclear GBPL circuit controls immunity in plants.

Authors - Shuai Huang
humanWB

Signal Transduct Target Ther

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion

Authors - Wenwen Zheng
humanWB

Cell

Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.

Authors - Xun Huang
mouseWB

Adv Mater

A Physiologically Responsive Nanocomposite Hydrogel for Treatment of Head and Neck Squamous Cell Carcinoma via Proteolysis-targeting Chimeras Enhanced Immunotherapy

Authors - Yaping Wu
humanWB

Nat Genet

N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.

Authors - Yuan Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wissem (Verified Customer) (06-27-2024)

Antibody works very well at detecting Histone H3 in extracts from U2OS, HCT116, HeLa, RPE1 and HEK293 cells - used at 1:10,000 to 1:15,000 incubated overnight at 4C

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10,000
FH

Roy (Verified Customer) (06-12-2024)

This antibody is great at detecting Histone H3 by WB (1/1000- 1h incubation at RT) with beautiful enrichment at the purified chromatin fraction.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa cells
FH

Mi (Verified Customer) (06-25-2023)

It works very well in nuclear extracts from human brown adipocyte cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: human brown adipocytes
FH

Veda (Verified Customer) (04-29-2022)

IF staining test in cryo sections of skin. It does not seem to work. Maybe it only stains the nuclear membrane.

  • Applications: Immunofluorescence
FH

Pradeep (Verified Customer) (09-19-2020)

Good antibody for Western blot. Selvaraju, V., Thirunavukkarasu, M., Joshi, M. et al. Deletion of newly described pro-survival molecule Pellino-1 increases oxidative stress, downregulates cIAP2/NF-κB cell survival pathway, reduces angiogenic response, and thereby aggravates tissue function in mouse ischemic models. Basic Res Cardiol 115, 45 (2020). https://doi.org/10.1007/s00395-020-0804-4

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Heart Tissue
FH

Tanusree (Verified Customer) (12-03-2019)

The antibody works great in Western blotting analysis.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain
FH

Ruiting (Verified Customer) (11-11-2019)

I use the Proteintech antibodies almost every week, the antibodies never failed to me. Very gald to use their products with a great price.Ruiting

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: Mouse testis and brains
FH

Robert (Verified Customer) (07-18-2019)

The Histon H3 antibody gives a signal in the three nuclear samples, but not in the cytoplasmic samples.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: ST cells (porcine)
Histone H3 Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:17168-1-AP)